Higuchi Yuzuru, Asaumi Jun-ichi, Murakami Jun, Matsuzaki Hidenobu, Wakasa Toru, Inoue Tetsuyoshi, Shigehara Hiroshi, Konouchi Hironobu, Hisatomi Miki, Kawasaki Shoji, Hiraki Yoshio, Kishi Kanji
Department of Oral and Maxillofacial Radiology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University, 2-5-1 Shikata-cho, Okayama 700-8525, Japan.
Oncol Rep. 2003 May-Jun;10(3):671-7.
We report herein the effects of p53 gene therapy in the radiotherapy or thermotherapy of eight human head and neck squamous cell carcinoma (SCC) cell lines. The discrepancy between radiosensitivity combined with p53 gene therapy than that without p53 gene therapy increased among the eight SCC cell lines. The discrepancy increased in the thermosensitivity at 43 degrees C and decreased in that at 44 degrees C among the eight SCC cell lines. Thus, the p53 gene therapy did not always improve the discrepancy between radiosensitivity and thermosensitivity in the eight SCC cell lines. In the radiotherapy combined with adenoviral p53 gene therapy, the survival rates of three of eight SCC cell lines decreased, and that of only one cell line increased compared with radiotherapy alone. In thermotherapy combined with p53 gene therapy, the survival rates of three at 44 degrees C and five at 43 degrees C of the eight SCC cell lines decreased, although only one cell line at 43 degrees C increased its survival rate compared with thermotherapy alone. The p53 gene therapy decreased the survival rates of both radiotherapy and thermotherapy in three of eight SCC cell lines. Further, the distribution of plots on the basis of the time for 10% survival of radiotherapy and the dose for 10% survival of thermotherapy with p53 gene therapy shifted to the lower left side of the plots compared with those without p53 gene therapy. These findings indicated that p53 gene therapy improves the effects of both radiotherapy and thermotherapy.
我们在此报告p53基因治疗对8种人头颈部鳞状细胞癌（SCC）细胞系放疗或热疗的影响。在这8种SCC细胞系中，联合p53基因治疗的放射敏感性与未联合p53基因治疗的放射敏感性之间的差异有所增加。在这8种SCC细胞系中，43℃时热敏感性差异增加，44℃时热敏感性差异减小。因此，p53基因治疗并非总能改善这8种SCC细胞系放射敏感性与热敏感性之间的差异。在联合腺病毒p53基因治疗的放疗中，8种SCC细胞系中有3种的生存率下降，与单纯放疗相比，只有1种细胞系的生存率增加。在联合p53基因治疗的热疗中，8种SCC细胞系中44℃时3种、43℃时5种的生存率下降，尽管43℃时只有1种细胞系与单纯热疗相比生存率增加。p53基因治疗使8种SCC细胞系中的3种放疗和热疗生存率均下降。此外，与未进行p53基因治疗的情况相比，基于p53基因治疗的放疗10%生存率时间和热疗10%生存率剂量绘制的图的分布向左下方移动。这些发现表明p53基因治疗可提高放疗和热疗的效果。
