Peng Ching-Tien, Chen Jui-Chang, Yeh Kun-Tu, Wang Yu-Feng, Hou Ming-Feng, Lee Tai-Ping, Shih Mu-Chih, Chang Jan-Yi, Chang Jan-Gowth
Department of Molecular Medicine and Pediatrics, China Medical College Hospital, Taichung, Taiwan, ROC.
Int J Mol Med. 2003 Jan;11(1):85-9.
Sulfotransferase (SULT) enzymes play an important role in the detoxification, metabolism and bioactivation of numerous xenobiotics, many dietary and environmental mutagens, drugs, neurotransmitters and hormones. The genes for SULT1A1 and SULT1A2 contain common genetic polymorphisms that are associated with individual variations in the level of enzyme activities as well as variations of biochemical and physical properties. We developed a PCR-RFLP method to analyze the frequencies of SULT1A1 and SULT1A2 alleles among cancerous patients and normal controls in Taiwan. The results showed that SULT1A11 and SULT1A21 were in positive linkage disequilibrium. Neither SULT1A13 nor SULT1A23 were found in this study. The frequencies of SULT1A12 and SULT1A22 for hepatic, colon, lung, oral, gastric, renal and cervical cancerous patients were 3.95, 5.56, 4.92, 3.84, 2.70, 7.41 and 4.50%, respectively. No statistical significance was found for these cancer patients after comparison with normal controls (4.0%) for the allelic frequencies of SULT1A12 and SULT1A22.
磺基转移酶（SULT）在众多外源性物质、许多饮食和环境诱变剂、药物、神经递质及激素的解毒、代谢和生物活化过程中发挥着重要作用。SULT1A1和SULT1A2基因存在常见的基因多态性，这些多态性与酶活性水平的个体差异以及生化和物理性质的变化相关。我们开发了一种聚合酶链反应 - 限制性片段长度多态性（PCR - RFLP）方法，用于分析台湾癌症患者和正常对照中SULT1A1和SULT1A2等位基因的频率。结果显示，SULT1A11和SULT1A21呈正连锁不平衡。本研究未发现SULT1A13和SULT1A23。肝癌、结肠癌、肺癌、口腔癌、胃癌、肾癌和宫颈癌患者中SULT1A12和SULT1A22的频率分别为3.95%、5.56%、4.92%、3.84%、2.70%、7.41%和4.50%。将这些癌症患者与正常对照（4.0%）的SULT1A12和SULT1A22等位基因频率进行比较后，未发现统计学意义。
