在正常和兴奋性毒性条件下，鸟苷可增强大脑皮质切片中谷氨酸的摄取。
Guanosine enhances glutamate uptake in brain cortical slices at normal and excitotoxic conditions.
作者信息
Frizzo Marcos Emílo dos Santos, Lara Diogo Rizzato, Prokopiuk Alexandre de Souza, Vargas Carmen Regla, Salbego Christianne Gazzana, Wajner Moacir, Souza Diogo Onofre
机构信息
Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
出版信息
Cell Mol Neurobiol. 2002 Jun;22(3):353-63. doi: 10.1023/a:1020728203682.
Abstract
- The effect of guanosine on L-[2,3-3H]glutamate uptake was investigated in brain cortical slices under normal or oxygen-glucose deprivation (OGD) conditions. 2. In slices exposed to physiological conditions, guanosine (1-100 microM) stimulated glutamate uptake (up to 100%) in a concentration-dependent manner when a high (100 microM) but not a low (1 microM) concentration of glutamate was used. 3. In slices submitted to OGD, guanosine 1 and 100 microM also increased 100 microM glutamate uptake (38 and 70%, respectively). 4. The increasing of glutamate and taurine released to the incubation medium in cortical slices submitted to OGD were significantly attenuated by the presence of guanosine in the incubation medium. 5. Guanosine prevented the increase in propidium iodide incorporation into cortical slices induced by OGD, indicating a protective role against ischemic injury. 6. These results support the hypothesis of a protective role for guanosine during brain ischemia, possibly by activating glutamate uptake into neural cells.
摘要
- 在正常或氧糖剥夺（OGD）条件下，研究了鸟苷对脑皮质切片中L-[2,3-³H]谷氨酸摄取的影响。2. 在处于生理条件的切片中，当使用高浓度（100μM）而非低浓度（1μM）的谷氨酸时，鸟苷（1 - 100μM）以浓度依赖性方式刺激谷氨酸摄取（高达100%）。3. 在经受OGD的切片中，1μM和100μM的鸟苷也增加了100μM谷氨酸的摄取（分别增加38%和70%）。4. 在经受OGD的皮质切片中，孵育培养基中鸟苷的存在显著减弱了释放到孵育培养基中的谷氨酸和牛磺酸的增加。5. 鸟苷可防止OGD诱导的碘化丙啶掺入皮质切片增加，表明其对缺血性损伤具有保护作用。6. 这些结果支持鸟苷在脑缺血期间具有保护作用的假说，可能是通过激活神经细胞对谷氨酸的摄取来实现的。
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