Department of Chemistry, University of Cincinnati, 404 Crosley Tower, 312 College Drive, Cincinnati, Ohio 45221-0172, United States.
ACS Chem Neurosci. 2023 May 3;14(9):1646-1658. doi: 10.1021/acschemneuro.3c00048. Epub 2023 Apr 11.
Guanosine acts in both neuroprotective and neurosignaling pathways in the central nervous system; in this paper, we present the first fast voltammetric measurements of endogenous guanosine release during pre- and post-ischemic conditions. We discuss the metric of our measurements via analysis of event concentration, duration, and interevent time of rapid guanosine release. We observe changes across all three metrics from our normoxic to ischemic conditions. Pharmacological studies were performed to confirm that guanosine release is a calcium-dependent process and that the signaling observed is purinergic. Finally, we show the validity of our ischemic model via staining and fluorescent imaging. Overall, this paper sets the tone for rapid monitoring of guanosine and provides a platform to investigate the extent to which guanosine accumulates at the site of brain injury, i.e., ischemia.
鸟苷在中枢神经系统的神经保护和神经信号通路中均有作用；在本文中，我们首次进行了快速伏安测量，以检测在缺血前和缺血后条件下内源性鸟苷的释放。我们通过对事件浓度、持续时间和快速鸟苷释放的事件间时间的分析来讨论我们测量的度量标准。我们观察到从常氧到缺血条件下，所有三个度量标准都发生了变化。进行了药理学研究以确认鸟苷释放是一个依赖于钙的过程，并且观察到的信号是嘌呤能的。最后，我们通过染色和荧光成像显示了我们的缺血模型的有效性。总的来说，本文为快速监测鸟苷奠定了基础，并提供了一个平台来研究鸟苷在脑损伤部位（即缺血）积累的程度。
