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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Tramontina Francine, Conte Sabrina, Gonçalves Daniela, Gottfried Carmem, Portela Luis V, Vinade Lucia, Salbego Christianne, Gonçalves Carlos-Alberto

Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.

Cell Mol Neurobiol. 2002 Jun;22(3):373-8. doi: 10.1023/a:1020732304591.

We investigated the content of S100B protein by ELISA in three brain regions (hippocampus, cerebral cortex, and cerebellum) and in cerebrospinal fluid of rats during postnatal development as well as the content and secretion of S100B in pre- and postconfluent primary astrocyte cultures. 2. An accumulation of S100B occurred in all brain regions with similar ontogenetic pattern between second and fourth postnatal weeks. However, we observed a decrease in the cerebrospinal fluid S100B after the critical period for synaptogenesis in rodents. 3. A similar profile of cell accumulation and decrease in basal secretion was also observed during aging of astrocyte cultures. 4. These data contribute to the proposal that S100B is an important glial-derived protein during brain development and that changes in extracellular levels of S100B may be related to glial proliferation and synaptogenesis.

我们采用酶联免疫吸附测定法（ELISA）研究了出生后发育过程中大鼠三个脑区（海马体、大脑皮层和小脑）以及脑脊液中S100B蛋白的含量，同时还研究了原代星形胶质细胞融合前和融合后培养物中S100B的含量及分泌情况。2. 出生后第二至第四周，所有脑区均出现S100B的积累，且其个体发育模式相似。然而，我们观察到啮齿动物突触发生关键期后，脑脊液中S100B含量下降。3. 在星形胶质细胞培养物老化过程中，也观察到了类似的细胞积累和基础分泌减少的情况。4. 这些数据支持了以下观点：S100B是大脑发育过程中一种重要的神经胶质衍生蛋白，S100B细胞外水平的变化可能与神经胶质细胞增殖和突触发生有关。

Tramontina Francine, Conte Sabrina, Gonçalves Daniela, Gottfried Carmem, Portela Luis V, Vinade Lucia, Salbego Christianne, Gonçalves Carlos-Alberto

Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.

Cell Mol Neurobiol. 2002 Jun;22(3):373-8. doi: 10.1023/a:1020732304591.

We investigated the content of S100B protein by ELISA in three brain regions (hippocampus, cerebral cortex, and cerebellum) and in cerebrospinal fluid of rats during postnatal development as well as the content and secretion of S100B in pre- and postconfluent primary astrocyte cultures. 2. An accumulation of S100B occurred in all brain regions with similar ontogenetic pattern between second and fourth postnatal weeks. However, we observed a decrease in the cerebrospinal fluid S100B after the critical period for synaptogenesis in rodents. 3. A similar profile of cell accumulation and decrease in basal secretion was also observed during aging of astrocyte cultures. 4. These data contribute to the proposal that S100B is an important glial-derived protein during brain development and that changes in extracellular levels of S100B may be related to glial proliferation and synaptogenesis.

我们采用酶联免疫吸附测定法（ELISA）研究了出生后发育过程中大鼠三个脑区（海马体、大脑皮层和小脑）以及脑脊液中S100B蛋白的含量，同时还研究了原代星形胶质细胞融合前和融合后培养物中S100B的含量及分泌情况。2. 出生后第二至第四周，所有脑区均出现S100B的积累，且其个体发育模式相似。然而，我们观察到啮齿动物突触发生关键期后，脑脊液中S100B含量下降。3. 在星形胶质细胞培养物老化过程中，也观察到了类似的细胞积累和基础分泌减少的情况。4. 这些数据支持了以下观点：S100B是大脑发育过程中一种重要的神经胶质衍生蛋白，S100B细胞外水平的变化可能与神经胶质细胞增殖和突触发生有关。