Biochemistry and Molecular Biology Laboratory, Department of Zoology, Banaras Hindu University, Varanasi, 221005 Uttar Pradesh, India.
Cell Mol Neurobiol. 2010 Jul;30(5):709-16. doi: 10.1007/s10571-009-9495-y. Epub 2010 Jan 23.
S100beta is a soluble calcium binding protein released by glial cells. It has been reported as a neurotrophic factor that promotes neurite maturation and outgrowth during development. This protein also plays a role in axonal stability and in long term potentiation in the adult brain. The ability of S100beta to modulate neuronal morphology raises the important question whether there is an age-related difference in the expression of S100beta in the cerebral and cerebellar cortices of AKR strain mice and is this change is region specific. Our RT-PCR and Western blotting experiments show that the expression of S100beta gene in the cerebral and cerebellar cortices starts from 0 day, peaks at about 45 days. However, in 70-week old mice its expression is significantly up-regulated as compared to that of 20-week old mice. S100beta follows the same age-related pattern in both cerebral and cerebellar cortices. These results suggest that S100beta is important for brain development and establishment of proper brain functions. Up-regulation of S100beta in old age may have some role in development of age-related pathological systems in the brain.
S100beta 是一种由神经胶质细胞释放的可溶性钙结合蛋白。它已被报道为一种神经营养因子，可在发育过程中促进神经突的成熟和生长。这种蛋白质在成年大脑中的轴突稳定性和长时程增强中也发挥作用。S100beta 调节神经元形态的能力提出了一个重要的问题，即在 AKR 品系小鼠的大脑和小脑皮质中，S100beta 的表达是否存在与年龄相关的差异，这种变化是否具有区域特异性。我们的 RT-PCR 和 Western blot 实验表明，S100beta 基因在大脑和小脑皮质中的表达从零天开始，在大约 45 天时达到峰值。然而，与 20 周龄的小鼠相比，70 周龄的小鼠中的表达显著上调。S100beta 在大脑和小脑皮质中呈现相同的与年龄相关的模式。这些结果表明，S100beta 对大脑发育和正常大脑功能的建立很重要。在老年时 S100beta 的上调可能在大脑中与年龄相关的病理系统的发展中起一定作用。
