Rashidan Kianoush Khajeh, Nassoury Nasha, Merzouki Abderrazzak, Guertin Claude
Institut National de la Recherche scientifique-Institut Armand-Frappier, Québec, Canada.
J Biochem Mol Biol. 2002 Nov 30;35(6):553-61. doi: 10.5483/bmbrep.2002.35.6.553.
A gene that encodes a protein homologue to baculoviral IE-1 was identified and sequenced in the genome of the Choristoneura fumiferana granulovirus (ChfuGV). The gene has an 1278 nucleotide (nt) open-reading frame (ORF) that encodes 426 amino acids with an estimated molecular weight of 50.33 kDa. At the nucleotide level, several cis-acting regulatory elements were detected within the promoter region of the ie-1 gene of ChfuGV along with other studied granuloviruses (GVs). Two putative CCAAT elements were detected within the noncoding leader region of this gene; one was located on the opposite strand at -92 and the other at -420 nt from the putative start triplet. Two baculoviral late promoter motifs (TAAG) were also detected within the promoter region of the ie-1 gene of ChfuGV. A single polyadenylation signal, AATAAA, was located 18nt downstream of the putative translational stop codon of ie-1 from ChfuGV. At the protein level, the amino acid sequence data that was derived from the nucleotide sequence in ChfuGV IE-1 was compared to those of the Cydia pomonella granulovirus (CpGV), Xestia c-nigrum granulovirus (XcGV) and Plutella xylostella granulovirus (PxGV). The C-terminal regions of the granuloviral IE-1 sequences appeared to be more conserved when compared to the N-terminal regions. A domain, similar to the basic helix-loop-helix like (bHLH-like) domain in NPVs, was detected at the C-terminal region of IE-1 from ChfuGV (residues 387 to 414). A phylogenetic tree for baculoviral IE-1 was constructed using a maximum parsimony analysis. A phylogenetic estimation demonstrates that ChfuGV IE-1 is most closely related to that of CpGV.
在云杉芽卷叶蛾颗粒体病毒（ChfuGV）的基因组中鉴定并测序了一个编码与杆状病毒IE-1蛋白同源物的基因。该基因有一个1278个核苷酸（nt）的开放阅读框（ORF），编码426个氨基酸，估计分子量为50.33 kDa。在核苷酸水平上，与其他已研究的颗粒体病毒（GVs）一样，在ChfuGV的ie-1基因启动子区域内检测到几个顺式作用调节元件。在该基因的非编码前导区域内检测到两个推定的CCAAT元件；一个位于相反链上距推定起始三联体-92 nt处，另一个位于-420 nt处。在ChfuGV的ie-1基因启动子区域内也检测到两个杆状病毒晚期启动子基序（TAAG）。一个单一的聚腺苷酸化信号AATAAA位于ChfuGV的ie-1推定翻译终止密码子下游18 nt处。在蛋白质水平上，将ChfuGV IE-1核苷酸序列推导的氨基酸序列数据与苹果蠹蛾颗粒体病毒（CpGV）、八字地老虎颗粒体病毒（XcGV）和小菜蛾颗粒体病毒（PxGV）的氨基酸序列数据进行了比较。与N端区域相比，颗粒体病毒IE-1序列的C端区域似乎更保守。在ChfuGV的IE-1的C端区域（第387至414位氨基酸残基）检测到一个与核型多角体病毒中类似碱性螺旋-环-螺旋（bHLH样）结构域相似的结构域。使用最大简约分析构建了杆状病毒IE-1的系统发育树。系统发育估计表明，ChfuGV IE-1与CpGV的IE-1关系最为密切。
