Zhang Xiaoxia, Liang Zhenpu, Yin Xinming, Wang Jianlin, Shao Xinfeng
College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, China,
Arch Virol. 2014 Jul;159(7):1869-72. doi: 10.1007/s00705-014-2001-y. Epub 2014 Feb 12.
The complete nucleotide sequence of Agrotis segetum granulovirus Shanghai strain (AgseGV-L1) was determined and compared with that of AgseGV Xinjiang strain (AgseGV-XJ). The circular genome of AgseGV-L1 is 131,442 bp and has a G+C content of 37.27 %. It includes 149 ORFs, 24 of which are unique to AgseGV (AgseGV-L1 and AgseGV-XJ [GenBank accession no. NC_005839]). The average level of amino acid sequence identity between AgseGV-L1 and other granulovirus (GV) homologues (except AgseGV-XJ) ranged from 44.3 % (Adoxophyes orana granulovirus [AdorGV]) to 49 % (Cydia pomonella granulovirus [CpGV]). The AgseGV-L1 genome is 99 % identical to that of AgseGV-XJ. They have 196 differences, including 172 substitutions, 21 deletions and 3 insertions. Two homologous regions (hrs) were detected in two intergenic spaces, which share low identity and both lack a palindromic core. A p6.9 gene was found in this genome, which shared 38 %-59 % amino acid identity with those of other baculoviruses. No differences were found in the hr and p6.9 sequences of AgseGV-L1 and AgseGV-XJ. Ie-1 is a known immediate-early gene, but AgseGV-L1 ie-1 has no recognizable promoter element. By BLASTP analysis, one bro gene homologue of NPVs was detected (Agse148). Phylogenetic analysis based on the 29 core baculovirus genes indicated that AgseGV-L1 is closely related to AgseGV-XJ, Helicoverpa armigera granulovirus (HearGV), Helicoverpa armigera granulovirus (XecnGV), Pseudaletia unipuncta granulovirus (PsunGV), Spodoptera litura granulovirus (SpliGV) and Plutella xylostella granulovirus (PlxyGV).
测定了小地老虎颗粒体病毒上海株（AgseGV-L1）的全核苷酸序列，并与小地老虎颗粒体病毒新疆株（AgseGV-XJ）进行了比较。AgseGV-L1的环状基因组为131,442 bp，G+C含量为37.27%。它包含149个开放阅读框（ORF），其中24个是AgseGV（AgseGV-L1和AgseGV-XJ [GenBank登录号：NC_005839]）所特有的。AgseGV-L1与其他颗粒体病毒（GV）同源物（除AgseGV-XJ外）的氨基酸序列平均同一性水平在44.3%（苹小卷叶蛾颗粒体病毒 [AdorGV]）至49%（苹果蠹蛾颗粒体病毒 [CpGV]）之间。AgseGV-L1基因组与AgseGV-XJ的基因组有99%的同一性。它们有196个差异，包括172个替换、21个缺失和3个插入。在两个基因间隔区检测到两个同源区域（hrs），它们的同一性较低，且都缺乏回文核心。在该基因组中发现了一个p6.9基因，它与其他杆状病毒的p6.9基因的氨基酸同一性为38%-59%。AgseGV-L1和AgseGV-XJ的hr和p6.9序列未发现差异。Ie-1是一个已知的立即早期基因，但AgseGV-L1的ie-1没有可识别的启动子元件。通过BLASTP分析，检测到一个NPVs的bro基因同源物（Agse148）。基于29个核心杆状病毒基因的系统发育分析表明，AgseGV-L1与AgseGV-XJ、棉铃虫颗粒体病毒（HearGV）、棉铃虫颗粒体病毒（XecnGV）、小地老虎颗粒体病毒（PsunGV）、斜纹夜蛾颗粒体病毒（SpliGV）和小菜蛾颗粒体病毒（PlxyGV）密切相关。
