Jang Sunphil, Yi Lee S H
Department of Biological Science, Sungkyunkwan University, Swuon 440-746, Korea.
J Biochem Mol Biol. 2002 Nov 30;35(6):604-8. doi: 10.5483/bmbrep.2002.35.6.604.
The effects of progesterone on the acrosome reaction, as well as the effects of RU486 on the progesterone-induced acrosome reaction in capacitated boar spermatozoa, were investigated. Progesterone, a major steroid that is secreted by the cumulus cells of oocyte, clearly induced the acrosome reaction in a dose-dependent manner in capacitated boar spermatozoa, even though it failed to show similar effects in non-capacitated spermatozoa. RU486, a potent antiprogestin, significantly reduced the effects of progesterone on the progesterone-induced acrosome reaction; however, when treated alone, it showed no inhibitory effects on the acrosome reaction. The inhibitory effects of RU486 were also shown to be dose dependent. These results imply that in addition to the wellknown inducer of the acrosome reaction, zona pellucida, progesterone can also induce the acrosome reaction through its specific receptors on spermatozoa after the spermatozoa undergo capacitation.
研究了孕酮对顶体反应的影响，以及RU486对孕酮诱导的获能公猪精子顶体反应的影响。孕酮是由卵母细胞的卵丘细胞分泌的一种主要甾体，即使在未获能的精子中未显示出类似作用，但在获能的公猪精子中，它能以剂量依赖的方式明显诱导顶体反应。强效抗孕激素RU486显著降低了孕酮对孕酮诱导的顶体反应的影响；然而，单独处理时，它对顶体反应无抑制作用。RU486的抑制作用也呈剂量依赖性。这些结果表明，除了众所周知的顶体反应诱导剂透明带外，孕酮在精子获能后也可通过其在精子上的特异性受体诱导顶体反应。
