Yang J, Serres C, Baulieu E E, Jouannet P
Laboratoire d'Histologie, Embryologie, Biologie de la Reproduction, Hôpital Cochin, Université Paris V, France.
Int J Androl. 1996 Feb;19(1):61-6. doi: 10.1111/j.1365-2605.1996.tb00436.x.
Previous studies have shown that RU486 decreases the concentration of intracellular calcium ([Ca2+]i) in human spermatozoa in vitro and partially antagonizes the effect of progesterone on calcium influx and sperm acrosome reaction. The present study has examined the effect of RU486 on the penetration of human spermatozoa into zona-free hamster oocytes. RU486 (10 microM) decreased significantly the rate of penetration of zona-free hamster oocytes by human spermatozoa, and nearly abolished penetration when present in the medium at concentrations of 50 and 100 microM. RU486 must be present in the medium to exert its inhibitory effect. At the same concentration (10 microM) as RU486, progesterone was unable to reverse the inhibitory effect of RU486 on the penetration rate of human spermatozoa.
先前的研究表明，米非司酮在体外可降低人类精子细胞内钙的浓度（[Ca2+]i），并部分拮抗孕酮对钙内流和精子顶体反应的影响。本研究检测了米非司酮对人类精子穿透去透明带仓鼠卵母细胞的影响。米非司酮（10微摩尔）显著降低了人类精子穿透去透明带仓鼠卵母细胞的速率，当培养基中米非司酮浓度为50和100微摩尔时，几乎完全抑制了穿透。米非司酮必须存在于培养基中才能发挥其抑制作用。在与米非司酮相同的浓度（10微摩尔）下，孕酮无法逆转米非司酮对人类精子穿透率的抑制作用。
