Weber Astrid, Maier Rolf F, Hoffmann Ulrike, Grips Martin, Hoppenz Marc, Aktas Ayse G, Heinemann Uwe, Obladen Michael, Schuchmann Sebastian
Department of Neonatology, Charité, Humboldt University Berlin, Campus Virchow-Klinikum, Augustenburger Platz 1, D-13353 Berlin, Germany.
Brain Res. 2002 Dec 27;958(2):305-11. doi: 10.1016/s0006-8993(02)03604-1.
Erythropoietin (EPO) prevents neuronal damage following ischemic, metabolic, and excitotoxic stress. In this study evoked extracellular field potentials (FP) were used to investigate the effect of EPO on synaptic transmission in hippocampal slice cultures. EPO treated cultured slices (40 units/ml for 48 h) showed significantly increased FP during and following oxygen and glucose deprivation compared with untreated control slices. The addition of the Jak2 inhibitor AG490 (50 microM for 48 h) blocked the EPO effect. These data suggest that EPO improves synaptic transmission during and following ischemia in hippocampal slice cultures.
促红细胞生成素（EPO）可预防缺血、代谢及兴奋性毒性应激后的神经元损伤。在本研究中，采用诱发细胞外场电位（FP）来研究EPO对海马切片培养物中突触传递的影响。与未处理的对照切片相比，经EPO处理的培养切片（40单位/毫升，处理48小时）在氧和葡萄糖剥夺期间及之后显示出显著增加的FP。添加Jak2抑制剂AG490（50微摩尔，处理48小时）可阻断EPO的作用。这些数据表明，EPO可改善海马切片培养物在缺血期间及之后的突触传递。
