促红细胞生成素促进新生卒中体外模型中海马神经发生。
Erythropoietin promotes hippocampal neurogenesis in in vitro models of neonatal stroke.
机构信息
Department of Neurology, University of California, San Francisco, CA 94143-0663, USA.
出版信息
Neurobiol Dis. 2010 May;38(2):259-65. doi: 10.1016/j.nbd.2010.01.015. Epub 2010 Feb 1.
Abstract
The hippocampus is often injured in neonatal stroke. We have investigated the effect of erythropoietin (EPO) on oxygen-glucose deprived hippocampal slices and hypoxic progenitor cells. EPO improved survival of the organotypic hippocampal slices with significantly less cell death in the dentate gyrus and an increased number of proliferating cells 4-5 days after insult. Significantly fewer markers of neurogenesis were seen after the insult but when EPO was added to the culture medium, neurogenesis was sustained. When hippocampal progenitor cultures were stimulated into differentiation, more cells chose a neuronal cell fate when treated with EPO. These findings support the hypothesis that EPO not only prevents ischemia induced cell death but promotes neuronal cell fate commitment in in vitro models of neonatal stroke.
摘要
海马体在新生儿中风中经常受损。我们研究了促红细胞生成素 (EPO) 对缺氧葡萄糖剥夺海马切片和缺氧祖细胞的影响。EPO 改善了器官型海马切片的存活，在损伤后 4-5 天，齿状回中的细胞死亡明显减少，增殖细胞的数量增加。损伤后神经发生的标志物明显减少，但当 EPO 添加到培养基中时，神经发生得到维持。当海马祖细胞培养物被刺激分化时，用 EPO 处理时，更多的细胞选择神经元细胞命运。这些发现支持这样的假设，即 EPO 不仅可以预防缺血诱导的细胞死亡，还可以促进体外新生儿中风模型中的神经元细胞命运决定。
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Erythropoietin promotes neuronal replacement through revascularization and neurogenesis after neonatal hypoxia/ischemia in rats.促红细胞生成素通过新生大鼠缺氧/缺血后的血管再生和神经发生促进神经元替代。
Stroke. 2007 Oct;38(10):2795-803. doi: 10.1161/STROKEAHA.107.483008. Epub 2007 Aug 16.
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Endogenous erythropoietin signaling is required for normal neural progenitor cell proliferation.正常神经祖细胞增殖需要内源性促红细胞生成素信号。
J Biol Chem. 2007 Aug 31;282(35):25875-83. doi: 10.1074/jbc.M701988200. Epub 2007 Jun 28.
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