Aldana Marcos Hernán Javier, Cintia Ferrari Carina, Cervino Claudio, Affanni Jorge Mario
Instituto de Neurociencia (INEUCI-CONICET), Universidad de Buenos Aires, Argentina.
Exp Eye Res. 2002 Dec;75(6):731-44. doi: 10.1006/exer.2002.2055.
The anatomical, histological, histochemical and ultrastructural characteristics of the lacrimal gland (LG) and nictitans gland (NG) of the armadillo Chaetophractus villosus were described. The histochemical and histological features of both glands in male and female adult animals were compared. The tissues were processed with conventional techniques for light and transmission electron microscopy. Fixed specimens were submitted to a battery of tests for glycans, glycosaminglycans, glycoconjugates, proteins, and lipids. The LG of the armadillo may be considered within the set of glandulae lacrimales superior in which primates, carnivores, perisodactyls and artiodactyls are included. The localization of the NG was similar to that of other mammals. Lacrimal and NG were histologically and histochemically identical. The secretory endpieces consisted of three cell types: (1) Mucous cells (MC) with different types of mucous secretory granules with neutral and sialic acid-containing glycoconjugates (GCs). (2)Seromucous cells (SMC) showing a variety of moderately electron dense secretory granules with flocculent material with carboxylated acidic, neutral, and sialic acid-containing GCs. Intercellular canaliculi with junctional complexes and basolateral intercellular spaces were frequent. (3) Serous cells (SC) with electron dense secretory granules. Histochemically, they showed the strongest reaction for proteins and neutral, weakly acid and carboxylated acidic GCs. The epithelium of the intra- and inter-lobular excretory ducts showed secretory activity, junctional complexes, and wide basolateral intercellular spaces with lateral folds. The endpieces and ducts were surrounded by myoepithelial cells. The stroma was characterized by fenestrated endothelium, unmyelinated axons, and abundant plasma cells. MC, SMC, and the duct system were richly innervated by hypolemmal nerve terminals.
描述了绒毛犰狳泪腺（LG）和瞬膜腺（NG）的解剖学、组织学、组织化学和超微结构特征。比较了成年雄性和雌性动物中这两种腺体的组织化学和组织学特征。采用常规技术对组织进行处理，用于光学显微镜和透射电子显微镜观察。将固定标本进行一系列针对聚糖、糖胺聚糖、糖缀合物、蛋白质和脂质的检测。犰狳的泪腺可被认为属于高级泪腺组，该组包括灵长类动物、食肉动物、奇蹄目动物和偶蹄目动物。瞬膜腺的定位与其他哺乳动物相似。泪腺和瞬膜腺在组织学和组织化学上相同。分泌末端由三种细胞类型组成：（1）黏液细胞（MC），具有不同类型的黏液分泌颗粒，含有中性和含唾液酸的糖缀合物（GCs）。（2）浆液黏液细胞（SMC），显示出各种中等电子密度的分泌颗粒，含有絮状物质，带有羧化酸性、中性和含唾液酸的GCs。具有连接复合体和基底外侧细胞间隙的细胞间小管很常见。（3）浆液细胞（SC），具有电子致密的分泌颗粒。在组织化学上，它们对蛋白质以及中性、弱酸和羧化酸性GCs显示出最强的反应。小叶内和小叶间排泄管的上皮显示出分泌活性、连接复合体以及带有侧褶的宽阔基底外侧细胞间隙。末端和导管被肌上皮细胞包围。基质的特征是有窗孔的内皮、无髓神经轴突和丰富的浆细胞。MC、SMC和导管系统由神经膜下神经末梢丰富地支配。
