Ramirez Maria Carmen, Sigal Luis J
Fox Chase Cancer Center, Philadelphia, PA 19111, USA.
J Immunol. 2002 Dec 15;169(12):6733-42. doi: 10.4049/jimmunol.169.12.6733.
Professional APCs (pAPC) can process and present on their own MHC class I molecules Ags acquired from Ag donor cells (ADC). This phenomenon of cross-presentation is essential in the induction of CD8(+) T cell responses to viruses that do not infect pAPC and possibly contributes to the induction of CD8(+) responses to many other viruses. However, little is known about the mechanisms underlying this process. In this study, we show that dendritic cells and macrophages cross-present a model Ag supplied by vaccinia virus-infected ADC via the cytosolic route. Strikingly, we also found that cross-presentation of Ags provided by vaccinia-infected cells occurs within a couple of hours of pAPC/ADC interaction, that the duration of cross-presentation lasts for only 16 h, and that cross-presentation can occur at early times of infection when the ADC are still alive.
专职抗原呈递细胞（pAPC）能够处理并在自身的MHC I类分子上呈递从抗原供体细胞（ADC）获取的抗原（Ag）。这种交叉呈递现象对于诱导CD8(+) T细胞对不感染pAPC的病毒产生应答至关重要，并且可能有助于诱导CD8(+) T细胞对许多其他病毒产生应答。然而，对于这一过程背后的机制知之甚少。在本研究中，我们表明树突状细胞和巨噬细胞通过胞质途径交叉呈递由痘苗病毒感染的ADC提供的模型抗原。令人惊讶的是，我们还发现痘苗感染细胞提供抗原的交叉呈递在pAPC/ADC相互作用后的几个小时内发生，交叉呈递的持续时间仅为16小时，并且当ADC仍然存活时，在感染早期就可以发生交叉呈递。
