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五种抗体作为细胞学标本中黑色素瘤诊断标志物的比较。

Comparison of five antibodies as markers in the diagnosis of melanoma in cytologic preparations.

作者信息

Sheffield Matthew V, Yee Herman, Dorvault Christine C, Weilbaecher Katherine N, Eltoum Isam A, Siegal Gene P, Fisher David E, Chhieng David C

机构信息

Department of Pathology, University of Alabama at Birmingham, 35249-6823, USA.

出版信息

Am J Clin Pathol. 2002 Dec;118(6):930-6. doi: 10.1309/EWK9-LUPR-6BC5-1GXV.

Abstract

We determined the sensitivity and specificity of 3 novel antibodies (microphthalmia transcription factor [Mitf], Melan-A, and tyrosinase) as markers for melanoma in cytologic preparations and compared the results with those of commonly used markers (S-100 protein [S-100] and HMB-45). We stained 72 cell blocks from 40 patients with melanoma and 32 with nonmelanocytic malignant neoplasms with antibodies against S-100, HMB-45, Mitf, Melan-A, and tyrosinase. Histologic correlation was available in more than 95% of cases. Nuclear stainingfor Mitf and cytoplasmic stainingfor S-100, HMB-45, Melan-A, and tyrosinase in more than 10% of tumor cells was considered positive. All 3 novel markers demonstrated sensitivity superior to S-100 and HMB-45. HMB-45, Melan-A, and Mitf demonstrated specificities of 97%. S-100 protein and tyrosinase were less specific. Sensitivity and specificity for the combination Mitf+/Melan-A+ were 95% and 100%, respectively, whereas they were 80% and 100%, respectively, for S-100+/HMB-45+. Mitf Melan-A, and tyrosinase are sensitive markersfor epithelioid melanoma. Mitf and Melan-A seem more specific than S-100 and tyrosinase. An antibody panel consisting of Mitf and Melan-A is superior to a panel of S-100 and HMB-45 in the diagnosis of melanoma in cytologic specimens.

摘要

我们测定了3种新型抗体(小眼畸形转录因子[Mitf]、黑色素A和酪氨酸酶)作为黑色素瘤细胞学标本标志物的敏感性和特异性,并将结果与常用标志物(S-100蛋白[S-100]和HMB-45)的结果进行比较。我们用抗S-100、HMB-45、Mitf、黑色素A和酪氨酸酶的抗体对40例黑色素瘤患者的72个细胞块和32例非黑素细胞性恶性肿瘤的细胞块进行染色。超过95%的病例有组织学相关性。肿瘤细胞中超过10%的Mitf核染色以及S-100、HMB-45、黑色素A和酪氨酸酶的胞质染色被视为阳性。所有3种新型标志物的敏感性均优于S-100和HMB-45。HMB-45、黑色素A和Mitf的特异性为97%。S-100蛋白和酪氨酸酶的特异性较低。Mitf+/黑色素A+组合的敏感性和特异性分别为95%和100%,而S-100+/HMB-45+组合的敏感性和特异性分别为80%和100%。Mitf、黑色素A和酪氨酸酶是上皮样黑色素瘤的敏感标志物。Mitf和黑色素A似乎比S-100和酪氨酸酶更具特异性。在细胞学标本中,由Mitf和黑色素A组成的抗体组合在黑色素瘤诊断方面优于由S-100和HMB-45组成的组合。

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