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通过定量蛋白质组学和免疫组织化学探究马黑素细胞肿瘤中的Wnt信号通路和功能信号

Probing Wnt pathway and functional signal in equine melanocytic neoplasms through quantitative proteomics and immunohistochemistry.

作者信息

Tesena Parichart, Vinijkumthorn Ruethaiwan, Kingkaw Amornthep, Yanyongsirikarn Petchpailin, Phasuk Khajornpol, Ploypetch Sekkarin, Phaonakrop Narumon, Roytrakul Sittiruk, Vongsangnak Wanwipa, Prapaiwan Nawarus

机构信息

Department of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand.

Faculty of Science, Interdisciplinary Graduate Program in Bioscience, Kasetsart University, Bangkok, 10900, Thailand.

出版信息

BMC Vet Res. 2025 Aug 7;21(1):509. doi: 10.1186/s12917-025-04956-w.

DOI:10.1186/s12917-025-04956-w
PMID:40775356
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12329947/
Abstract

BACKGROUND

Equine melanocytic neoplasm (EMN) is a skin tumor commonly observed in grey horses. Limited research has yet to investigate proteomic profiles of EMN, particularly in the early stages and their expression patterns. This study, therefore, aimed to identify signature proteins from tissue biopsies to distinguish early EMN, severe EMN, and normal groups.

RESULTS

Using proteomic analysis of 19 tissue samples (normal: n = 6, early EMN: n = 7, severe EMN: n = 6) through LC-MS/MS, 12,310 proteins were identified. Differentially expressed proteins (DEPs) and functional interaction analysis revealed significant overexpression of Wnt signature proteins, e.g., canonical (Wnt2B) and non-canonical (Wnt5B) Wnt signaling in early EMN stages. Immunohistochemical staining (IHC) towards immunolocalizing Wnt signature protein, particularly the Wnt2B functional signal, further verified its higher expression in early EMN compared to other groups.

CONCLUSIONS

These findings suggest that the Wnt pathway and functional insight are key mediators in signal transduction during early EMN, offering potential markers for initial stage detection. This study enhances the understanding of EMN mechanisms and the role of Wnt proteins, with implications for developing future diagnostic and therapeutic strategies.

摘要

背景

马黑素细胞肿瘤（EMN）是灰马中常见的一种皮肤肿瘤。目前针对EMN蛋白质组学特征的研究有限，尤其是在早期阶段及其表达模式方面。因此，本研究旨在从组织活检中鉴定标志性蛋白质，以区分早期EMN、重度EMN和正常组。

结果

通过液相色谱-串联质谱（LC-MS/MS）对19个组织样本（正常组：n = 6，早期EMN组：n = 7，重度EMN组：n = 6）进行蛋白质组学分析，共鉴定出12310种蛋白质。差异表达蛋白（DEPs）和功能相互作用分析显示，在早期EMN阶段，Wnt标志性蛋白，如经典（Wnt2B）和非经典（Wnt5B）Wnt信号通路显著过表达。针对Wnt标志性蛋白，特别是Wnt2B功能信号进行免疫定位的免疫组织化学染色（IHC）进一步证实，与其他组相比，其在早期EMN中的表达更高。

结论

这些发现表明，Wnt信号通路及其功能洞察是早期EMN信号转导的关键介质，为早期检测提供了潜在的标志物。本研究增进了对EMN机制以及Wnt蛋白作用的理解，对未来诊断和治疗策略的开发具有重要意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6b2/12329947/01c8e457fe41/12917_2025_4956_Fig9_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6b2/12329947/01c8e457fe41/12917_2025_4956_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6b2/12329947/a97b86105e5c/12917_2025_4956_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6b2/12329947/62c40ceb8f46/12917_2025_4956_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6b2/12329947/e259f4c4c8fe/12917_2025_4956_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6b2/12329947/2ab4a3f5a149/12917_2025_4956_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6b2/12329947/689cdef86769/12917_2025_4956_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6b2/12329947/c0695e3fedc4/12917_2025_4956_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6b2/12329947/ed4e6451c155/12917_2025_4956_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6b2/12329947/01c8e457fe41/12917_2025_4956_Fig9_HTML.jpg

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3
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4
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5
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