Epstein E, Zak B, Baginski E S, Civin W H
Ann Clin Lab Sci. 1976 Jan-Feb;6(1):27-37.
The use of polyacrylamide gel electrophoresis (PAGE) for the separation of proteins in cerebrospinal fluid (CSF) results in greater definition than does a "routine" method such as cellulose acetate electrophoresis. Unconcentrated CSF is easily separated into as many as 18 bands by the use of PAGE. By means of a modified PAGE method described in this paper, unconcentrated and untreated CSF is quickly and conveniently analyzed for protein constituents. This modification involves a continuous buffer environment, a pore-size concentration gradient and CSF in amounts of 0.1 to 0.4 ml. Sucrose addition is not necessary in this procedure. Whereas most central nervous system (CNS) disease states do not yield consistently distinctive protein patterns, some diseases, such as vascular disease, infectious meningitis and some metastatic tumors, yield significantly altered patterns. It is suggested that the chief value of CSF protein electrophoresis at the present time is to follow the course of a CNS disease.
与“常规”方法（如醋酸纤维素电泳）相比，使用聚丙烯酰胺凝胶电泳（PAGE）分离脑脊液（CSF）中的蛋白质能得到更清晰的结果。通过PAGE，未浓缩的脑脊液很容易分离出多达18条带。采用本文所述的改良PAGE方法，可快速、便捷地分析未浓缩且未经处理的脑脊液中的蛋白质成分。这种改良包括连续缓冲环境、孔径浓度梯度以及0.1至0.4毫升的脑脊液用量。在此过程中无需添加蔗糖。虽然大多数中枢神经系统（CNS）疾病状态不会产生始终独特的蛋白质图谱，但某些疾病，如血管疾病、感染性脑膜炎和一些转移性肿瘤，会产生明显改变的图谱。目前认为，脑脊液蛋白质电泳的主要价值在于跟踪中枢神经系统疾病的病程。
