Cullis P A, Townsend L, LeWitt P, Pomara N, Reitz D
Department of Neurology, Wayne State University School of Medicine, Detroit, Michigan.
Mov Disord. 1986;1(3):179-86. doi: 10.1002/mds.870010303.
Protein patterns of cerebrospinal fluid (CSF) from patients with spasmodic torticollis (ST) were investigated to determine whether abnormalities previously reported could be detected and further identified. CSF was collected from 12 patients with ST and 6 normal controls. The CSF proteins were analyzed using sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis and silver staining. In 11 of the 12 patients with ST, a CSF protein pattern was observed which differed from that in the controls. The identity of the abnormal proteins was ascertained by blotting and immunostaining with specific antisera to IgG and ceruloplasmin (Cp). CSF from 2 of 12 patients had distinct bands staining for IgG and 7 had abnormal immunostaining for Cp.
对痉挛性斜颈（ST）患者脑脊液（CSF）的蛋白质模式进行了研究，以确定之前报道的异常情况是否能够被检测到并进一步识别。从12例ST患者和6名正常对照者中采集脑脊液。使用十二烷基硫酸钠（SDS）聚丙烯酰胺凝胶电泳和银染法分析脑脊液蛋白质。在12例ST患者中的11例中，观察到一种与对照者不同的脑脊液蛋白质模式。通过用针对IgG和铜蓝蛋白（Cp）的特异性抗血清进行印迹和免疫染色来确定异常蛋白质的身份。12例患者中有2例的脑脊液有IgG染色的明显条带，7例有Cp的异常免疫染色。
