Luo Yongquan, Cai Jingli, Liu Ying, Xue Haipeng, Chrest Francis J, Wersto Robert P, Rao Mahendra
Laboratory of Neurosciences, Gerontology Research Center, National Institute on Aging, Room 4E02, 5600 Nathan Shock Drive, Baltimore, Maryland 21224, USA.
J Neurochem. 2002 Dec;83(6):1481-97. doi: 10.1046/j.1471-4159.2002.01260.x.
To access and compare gene expression in fetal neuroepithelial cells (NEPs) and progenitor cells, we have used microarrays containing approximately 500 known genes related to cell cycle regulation, apoptosis, growth and differentiation. We have identified 152 genes that are expressed in NEPs and 209 genes expressed by progenitor cells. The majority of genes (141) detected in NEPs are also present in progenitor populations. There are 68 genes specifically expressed in progenitors with little or no expression in NEPs, and a few genes that appear to be present exclusively in NEPs. Using cell sorting, RT-PCR, in situ hybridization or immunocytochemistry, we have examined the segregation of expression to neuronal and glial progenitors, and identified several that appeared to be enriched in neuronal (e.g. CDK5, neuropilin, EphrinB2, FGF11) or glial (e.g. CXCR4, RhoC, CD44, tenascin C) precursors. Our data provide a first report of gene expression profiles of neural stem and progenitor cells at early stages of development, and provide evidence for the potential roles of specific cell cycle regulators, chemokines, cytokines and extracellular matrix molecules in neural development and lineage segregation.
为了检测和比较胎儿神经上皮细胞（NEP）和祖细胞中的基因表达，我们使用了包含约500个与细胞周期调控、凋亡、生长和分化相关的已知基因的微阵列。我们鉴定出152个在NEP中表达的基因和209个由祖细胞表达的基因。在NEP中检测到的大多数基因（141个）也存在于祖细胞群体中。有68个基因在祖细胞中特异性表达，在NEP中很少或没有表达，还有一些基因似乎仅存在于NEP中。通过细胞分选、逆转录-聚合酶链反应（RT-PCR）、原位杂交或免疫细胞化学，我们研究了基因表达在神经元和胶质祖细胞中的分离情况，并鉴定出了几个在神经元（如细胞周期蛋白依赖性激酶5（CDK5）、神经纤毛蛋白、EphrinB2、成纤维细胞生长因子11（FGF11））或胶质（如CXC趋化因子受体4（CXCR4）、RhoC、CD44、腱生蛋白C）前体细胞中似乎富集的基因。我们的数据首次报道了神经干细胞和祖细胞在发育早期的基因表达谱，并为特定细胞周期调节因子、趋化因子、细胞因子和细胞外基质分子在神经发育和谱系分离中的潜在作用提供了证据。
