Mittendorfer Bettina, Patterson Bruce W, Klein Samuel, Sidossis Labros S
Department of Internal Medicine and Center for Human Nutrition, Washington University School of Medicine, St Louis, Missouri 63110, USA.
Am J Physiol Endocrinol Metab. 2003 Apr;284(4):E708-15. doi: 10.1152/ajpendo.00411.2002. Epub 2002 Dec 10.
We have previously shown that sex and obesity independently affect basal very low density lipoprotein (VLDL)-triglyceride (TG) kinetics. In the present study, we investigated the effect of hyperglycemia-hyperinsulinemia on VLDL-TG kinetics in lean and obese men and women (n = 6 in each group). VLDL-TG kinetics were measured during basal, postabsorptive conditions and during glucose infusion (5.5 mg x kg FFM(-1) x min(-1)) by using [(2)H(5)]glycerol bolus injection in conjunction with compartmental modeling analysis. Basal VLDL-TG secretion in plasma was greater in obese than in lean men (7.8 +/- 0.6 and 2.9 +/- 0.4 micromol x l plasma(-1) x min(-1); P < 0.001) but was not different in lean and obese women (5.0 +/- 1.1 and 5.9 +/- 1.1 micromol x l plasma(-1) x min(-1)). Glucose infusion decreased the VLDL-TG secretion rate by approximately 50% in lean and obese men and in lean women (to 1.5 +/- 0.4, 4.0 +/- 0.6, and 2.2 +/- 0.4 micromol x l plasma(-1) x min(-1), respectively; all P < 0.05) but had no effect on the VLDL-TG secretion rate in obese women (4.9 +/- 1.0 micromol x l plasma(-1) x min(-1)). These results demonstrate that both sex and adiposity affect the regulation of VLDL-TG metabolism. Glucose and insulin decrease VLDL-TG production in both lean men and lean women; obesity is associated with resistance to the glucose- and insulin-mediated suppression of VLDL-TG secretion in women, but not in men.
我们之前已经表明，性别和肥胖各自独立影响基础极低密度脂蛋白（VLDL）-甘油三酯（TG）的动力学。在本研究中，我们调查了高血糖-高胰岛素血症对瘦和肥胖男性及女性（每组n = 6）VLDL-TG动力学的影响。通过使用[（2）H（5）]甘油推注注射结合房室模型分析，在基础、吸收后状态以及葡萄糖输注期间（5.5 mg×kg无脂体重（FFM）-1×min-1）测量VLDL-TG动力学。肥胖男性血浆中基础VLDL-TG分泌高于瘦男性（7.8±0.6和2.9±0.4 μmol×l血浆-1×min-1；P < 0.001），但瘦和肥胖女性之间无差异（5.0±1.1和5.9±1.1 μmol×l血浆-1×min-1）。葡萄糖输注使瘦和肥胖男性以及瘦女性的VLDL-TG分泌率降低约50%（分别降至1.5±0.4、4.0±0.6和2.2±0.4 μmol×l血浆-1×min-1；所有P < 0.05），但对肥胖女性的VLDL-TG分泌率无影响（4.9±1.0 μmol×l血浆-1×min-1）。这些结果表明，性别和肥胖均影响VLDL-TG代谢的调节。葡萄糖和胰岛素可降低瘦男性和瘦女性的VLDL-TG生成；肥胖与女性对葡萄糖和胰岛素介导的VLDL-TG分泌抑制的抵抗有关，但男性并非如此。
