Mittendorfer Bettina, Patterson Bruce W, Klein Samuel
Department of Internal Medicine and Center for Human Nutrition, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Am J Physiol Endocrinol Metab. 2003 Mar;284(3):E549-56. doi: 10.1152/ajpendo.00379.2002. Epub 2002 Dec 10.
The effects of obesity and weight loss on lipoprotein kinetics were evaluated in six lean women [body mass index (BMI): 21 +/- 1 kg/m(2)] and seven women with abdominal obesity (BMI: 36 +/- 1 kg/m(2)). Stable isotope tracer techniques, in conjunction with compartmental modeling, were used to determine VLDL-triglyceride (TG) and apolipoprotein B-100 (apoB-100) secretion rates in lean women and in obese women before and after 10% weight loss. VLDL-TG and VLDL-apoB-100 secretion rates were similar in lean and obese women. Weight loss decreased the rate of VLDL-TG secretion by approximately 40% (from 0.41 +/- 0.05 to 0.23 +/- 0.03 micromol x kg fat-free mass(-1) x min(-1); P < 0.05). The relative decline in VLDL-TG produced from nonsystemic fatty acids, derived from intraperitoneal and intrahepatic TG, was greater (61 +/- 7%) than the decline in VLDL-TG produced from systemic fatty acids, predominantly derived from subcutaneous TG (25 +/- 8%; P < 0.05). Weight loss did not affect VLDL-apoB-100 secretion rate. We conclude that weight loss decreases the rate of VLDL-TG secretion in women with abdominal obesity, primarily by decreasing the availability of nonsystemic fatty acids. There is a dissociation in the effect of weight loss on VLDL-TG and apoB-100 metabolic pathways that may affect VLDL particle size.
评估了肥胖和体重减轻对脂蛋白动力学的影响，研究对象为6名体重正常的女性[体重指数（BMI）：21±1kg/m²]和7名腹部肥胖女性（BMI：36±1kg/m²）。采用稳定同位素示踪技术结合房室模型，测定体重正常女性以及肥胖女性在体重减轻10%前后的极低密度脂蛋白甘油三酯（TG）和载脂蛋白B-100（apoB-100）分泌率。体重正常和肥胖女性的极低密度脂蛋白TG和极低密度脂蛋白apoB-100分泌率相似。体重减轻使极低密度脂蛋白TG分泌率降低约40%（从0.41±0.05降至0.23±0.03μmol×kg去脂体重⁻¹×min⁻¹；P<0.05）。由腹膜内和肝内TG衍生的非系统性脂肪酸产生的极低密度脂蛋白TG的相对下降幅度（61±7%）大于主要由皮下TG衍生的系统性脂肪酸产生的极低密度脂蛋白TG的下降幅度（25±8%；P<0.05）。体重减轻不影响极低密度脂蛋白apoB-100分泌率。我们得出结论，体重减轻可降低腹部肥胖女性的极低密度脂蛋白TG分泌率，主要是通过减少非系统性脂肪酸的可用性实现的。体重减轻对极低密度脂蛋白TG和apoB-100代谢途径的影响存在分离，这可能会影响极低密度脂蛋白颗粒大小。
