大鼠氯白血病组蛋白H2B的制备电泳纯化及特性分析

Purification by preparative electrophoresis and characterization of histone H2B from rat chloroleukaemia.

作者信息

Martinage A, Sautière P, Kerckaert J P, Biserte G

出版信息

Biochim Biophys Acta. 1976 Jan 20;420(1):37-41. doi: 10.1016/0005-2795(76)90342-1.

Abstract

Highly purified histone H2B from rat chloroleukaemia has been isolated by preparative electrophoresis at pH 2.7 in polyacrylamide slab gel, using the fraction F2b of Johns (Johns E. W. (1964) Biochem, J. 92, 55-59) as starting material. This histone was characterized by amino acid analysis and end groups determination. Comparative studies with homologous calf thymus histone show similarity of the amino acid compositions and of the amino terminal groups. the carboxyl terminal sequence presents two conservative substitutions.

摘要

以约翰斯（约翰斯 E.W.（1964 年），《生物化学杂志》92 卷，55 - 59 页）的 F2b 级分作为起始材料，通过在 pH 2.7 的聚丙烯酰胺平板凝胶中进行制备电泳，从大鼠氯白血病中分离出了高度纯化的组蛋白 H2B。通过氨基酸分析和末端基团测定对该组蛋白进行了表征。与同源小牛胸腺组蛋白的比较研究表明，氨基酸组成和氨基末端基团具有相似性。羧基末端序列存在两个保守性取代。