牛胰富含赖氨酸组蛋白的异质性研究。
Studies on heterogeneity of the lysine-rich histone from ox pancreas.
作者信息
Lipińska A, Kłyszejko-Stefanowicz L
出版信息
Acta Biochim Pol. 1978;25(2):157-62.
PMID:569416
Abstract
- Histone H1 from ox pancrease has been isolated by preparative electrophoresis at pH 2.7 in polyacrylamide slab gel, using the fraction F1 of Oliver et al. (1972, Biochem. J., 129, 349--353) as starting material. 2. The isolated histone H1 showed higher heterogeneity on isoelectric focusing than on polyacrylamide electrophoresis in long gel. The isoelectric points of the main subfractions of histone H1 were at pH 8.0--8.4.
摘要
- 以奥利弗等人（1972年，《生物化学杂志》，第129卷，349 - 353页）的F1级分为起始材料，通过在pH 2.7的聚丙烯酰胺平板凝胶中进行制备电泳，从牛胰腺中分离出组蛋白H1。2. 分离出的组蛋白H1在等电聚焦上显示出比在长凝胶中的聚丙烯酰胺电泳更高的异质性。组蛋白H1主要亚组分的等电点在pH 8.0 - 8.4。
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