高尔基体通过Rab34与Rab相互作用溶酶体蛋白的相互作用对溶酶体定位进行细胞器间调控。
Interorganellar regulation of lysosome positioning by the Golgi apparatus through Rab34 interaction with Rab-interacting lysosomal protein.
作者信息
Wang Tuanlao, Hong Wanjin
机构信息
Membrane Biology Laboratory, Institute of Molecular and Cell Biology, Singapore 117609, Singapore.
出版信息
Mol Biol Cell. 2002 Dec;13(12):4317-32. doi: 10.1091/mbc.e02-05-0280.
Abstract
We present evidence to suggest the existence of a regulatory pathway for the Golgi apparatus to modulate the spatial positioning of otherwise distantly located lysosomes. Rab34, a new member of the Rab GTPase family, is associated primarily with the Golgi apparatus. Expression of wild-type or GTP-restricted but not GDP-restricted versions of Rab34 causes spatial redistribution of lysosomes from the periphery to the peri-Golgi region. The regulation of lysosomal positioning by Rab34 depends on its association with the membrane mediated by prenylation and its direct interaction with Rab-interacting lysosomal protein (RILP). This biological activity, mediated by Rab34-RILP interaction, is dependent on Lys82 in the switch I region. Our results have uncovered a novel mechanism for the Golgi apparatus to regulate the spatial distribution of another organelle.
摘要
我们提供的证据表明存在一种调节途径，通过该途径高尔基体可调节原本位于远处的溶酶体的空间定位。Rab34是Rab GTPase家族的新成员，主要与高尔基体相关。野生型或GTP受限型（而非GDP受限型）Rab34的表达会导致溶酶体从细胞周边向高尔基体周围区域发生空间重新分布。Rab34对溶酶体定位的调节取决于其通过异戊二烯化介导的与膜的结合以及与Rab相互作用溶酶体蛋白（RILP）的直接相互作用。这种由Rab34-RILP相互作用介导的生物学活性依赖于开关I区域中的赖氨酸82。我们的研究结果揭示了高尔基体调节另一种细胞器空间分布的新机制。
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