Komatsu M, Murakami S
Biochim Biophys Acta. 1976 Jan 15;423(1):103-10. doi: 10.1016/0005-2728(76)90104-3.
ATP and pyrophosphate at high concentration (greater than 1 mM) inhibited photophosphorylation of isolated spinach chloroplasts in the normal salt medium and did not cause stimulation of electron transport. The inhibition of photophosphorylation by ATP or pyrophosphate was shown to be abolished by the addition of excess MgCl2, ADP and phosphate. It has been demonstrated that the rates of photophosphorylation in the absence and presence of ATP or pyrophosphate are determined similarly by the concentrations of magnesium-ADP (Mg - ADP-) and magnesiumphosphate (Mg - Pi) complexes. It is highly probable that Mg - ADP- and Mg - Pi, but not free ADP and free phosphate, are the active form of the substrates of photophosphorylation. This is in support of the view that ATP inhibits photophosphorylation by decreasing the concentration of Mg2+ which is available for the formation of the complex with ADP and phosphate.
高浓度（大于1 mM）的ATP和焦磷酸抑制了正常盐培养基中分离的菠菜叶绿体的光合磷酸化作用，且未引起电子传递的刺激。已表明，添加过量的MgCl₂、ADP和磷酸盐可消除ATP或焦磷酸对光合磷酸化的抑制作用。已经证明，在不存在和存在ATP或焦磷酸的情况下，光合磷酸化的速率同样由镁 - ADP（Mg - ADP⁻）和镁 - 磷酸盐（Mg - Pi）复合物的浓度决定。极有可能的是，Mg - ADP⁻和Mg - Pi而非游离的ADP和游离的磷酸盐是光合磷酸化底物的活性形式。这支持了以下观点：ATP通过降低可用于与ADP和磷酸盐形成复合物的Mg²⁺浓度来抑制光合磷酸化。
