Schreiber U, Colbow K, Vidaver W
Biochim Biophys Acta. 1976 Feb 16;423(2):249-63. doi: 10.1016/0005-2728(76)90183-3.
A newly observed general chlorophyll fluorescence induction effect in plants is described. Fluorescence yield can rise through as many as four different phases (alpha, beta, gamma, ) in the dark, when intact cells or leaves are rapidly heated (within approx. 2.5 s) from 20 to 40-50 degrees C. An analysis of this temperature-jump fluorescence induction in Scenedesmus obliquus leads to the following: 1. Phase alpha is due to removal of S-quenching and appears to be related to heat deactivation of the water-splitting enzyme system. With prolonged heating, irreversibility of alpha upon recooling reflects irreversible damage to the water-splitting enzyme system. 2. beta is independent of the S-states and of the redox state of primary System II acceptor Q. It is suggested that beta parallels functional separation of Q from the System II trapping centre. This effect is highly reversible. 3. gamma and beta reflect reduction of primary System II acceptor Q by a heat-induced endogenous reductant, which is probably identical to hydrogenase. Critical temperatures for pronounced alpha and beta phases differ markedly in different plants. Possible correlations between temperature-jump fluorescence inductio, thylakoid membrane lipid composition, lipid phase transition and lipid-protein interactions are discussed.
本文描述了一种新观察到的植物中普遍存在的叶绿素荧光诱导效应。当完整的细胞或叶片在大约2.5秒内从20℃迅速加热到40 - 50℃时，荧光产量在黑暗中可通过多达四个不同阶段（α、β、γ）上升。对斜生栅藻中这种温度跃升荧光诱导的分析得出以下结果：1. α阶段是由于S型猝灭的消除，似乎与水裂解酶系统的热失活有关。随着加热时间延长，再冷却时α阶段的不可逆性反映了水裂解酶系统的不可逆损伤。2. β阶段与S态以及初级光系统II受体Q的氧化还原状态无关。有人认为β阶段与Q从光系统II捕获中心的功能分离平行。这种效应是高度可逆的。3. γ阶段和β阶段反映了由热诱导的内源性还原剂对初级光系统II受体Q的还原，该还原剂可能与氢化酶相同。不同植物中明显的α阶段和β阶段的临界温度有显著差异。文中讨论了温度跃升荧光诱导、类囊体膜脂质组成、脂质相变和脂蛋白相互作用之间可能的相关性。
