Strasser R J, Butler W L
Biochim Biophys Acta. 1976 Dec 6;449(3):412-9. doi: 10.1016/0005-2728(76)90152-3.
Fluorescence and energy transfer properties of bean leaves greened by brief, repetitive xenon flashes were studied at -196 degrees C. The bleaching of P-700 has no influence on the yield of fluorescence at any wavelength of emission. The light-induced fluorescence yield changes which are observed in both the 690 and 730 nm emission bands in the low temperature fluorescence spectra are due to changes in the state of the Photosystem II reaction centers. The fluorescence yield changes in the 730 nm band are attributed to energy transfer from Photosystem II to Photosystem I. Such energy transfer was also confirmed by measurements of the rate of photooxidation of P-700 AT -196 degrees C in leaves in which the Photosystem II reaction centers were either all open or all closed. It is concluded that energy transfer from Photosystem II to Photosystem I occurs in the flashed bean leaves which lack the light-harvesting chlorophyll a/b protein.
在-196℃下研究了经短暂、重复氙闪光绿化的豆叶的荧光和能量转移特性。P-700的光漂白对任何发射波长下的荧光产率均无影响。在低温荧光光谱中690和730nm发射带中观察到的光诱导荧光产率变化是由于光系统II反应中心状态的改变。730nm波段的荧光产率变化归因于从光系统II到光系统I的能量转移。通过测量光系统II反应中心全部开放或全部关闭的叶片在-196℃下P-700的光氧化速率，也证实了这种能量转移。得出的结论是，在缺乏光捕获叶绿素a/b蛋白的闪光豆叶中发生了从光系统II到光系统I的能量转移。
