Dill K, Shafer R H
Biophys Chem. 1976 Jan;4(1):51-4. doi: 10.1016/0301-4622(76)80006-3.
We have previously shown that DNA will migrate radially inward in a concentric-cylinder shear flow apparatus. We assumed gaussian chain statistics, and we considered only linear molecules. In this paper, we extend the analysis to closed circular molecules, and we consider non-gaussian statistics for both linears and circles. We find that, in good solvents, the inward radial migration velocity is more sensitive to the molecular weight than M5/2, which we previously reported for gaussian chains. Furthermore, linears migrate radially inward 8 times faster than do circles of the same molecular weight. This suggests the possibility of separating linear from circular DNA in solution.
我们之前已经表明，在同心圆柱剪切流装置中，DNA会沿径向向内迁移。我们假设了高斯链统计，并仅考虑了线性分子。在本文中，我们将分析扩展到闭环圆形分子，并同时考虑了线性分子和圆形分子的非高斯统计。我们发现，在良溶剂中，向内的径向迁移速度对分子量的敏感度高于我们之前报道的高斯链的M5/2。此外，相同分子量的线性分子的径向向内迁移速度比圆形分子快8倍。这表明在溶液中分离线性DNA和圆形DNA是有可能的。
