一种用于分离非常大的DNA分子的流变学分离器。
A rhelogical separator for very large DNA molecules.
作者信息
Dill K A, Zimm B H
出版信息
Nucleic Acids Res. 1979 Oct 10;7(3):735-49. doi: 10.1093/nar/7.3.735.
Abstract
We present a rheological separation method for DNA molecules in which their deformability is used to advantage. This is the "radial migration method"; here we present experimental verification of the principle, theory having been reported elsewhere. The main conclusions are: (1) the theory is reasonably good; (2) radial migration is highly sensitive to the molecular weight, as predicted, and (3) intact T2 DNA (1.25 X 108 daltons) can be made to migrate about three centimeters in less than three hours.
摘要
我们提出了一种利用DNA分子可变形性的流变分离方法。这就是“径向迁移法”；在此我们给出该原理的实验验证，理论已在其他地方报道。主要结论如下：（1）该理论相当不错；（2）如所预测的，径向迁移对分子量高度敏感，以及（3）完整的T2 DNA（1.25×10^8道尔顿）能够在不到三小时内迁移约三厘米。
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