Meuret G, Fopp M, de Fliedner V, Senn H
Blut. 1976 Feb;32(2):79-86. doi: 10.1007/BF00995935.
More than 10(10) viable granulocytes are necessary for a therapeutical effective granulocyte transfusion. This number of cells can be harvested from normal donors by two techniques basing on different principles: continuous flow centrifugation (CFC) and filtration leucapheresis (FL). Our studies demonstrated that, under certain special conditions, the separation potentials of both methods are comparable yielding 2.5 to 3.0 X 10(10) granulocytes within 4 hrs. Granulocyte collection rate was optimal if donors were treated with dexamethasone during 16 hrs prior to the state of the procedure. However, the costs of CFC exceed those of FL by a factor of about two. The increased occurrence of side effects attributed to the transfusion of FL-granulocytes can be reduced to the level of CFC-granulocytes by repetitive filtration-elution leucapheresis minimizing cell damage. The studies define the efficiency spectrum of CFC which in addition to granulocyte separation includes collection of thrombocytes, cells for immunotherapy, and plasmapheresis.
治疗有效的粒细胞输注需要超过10×10⁹个存活的粒细胞。可以通过基于不同原理的两种技术从正常供体中采集到这个细胞数量：连续流动离心法（CFC）和过滤白细胞单采术（FL）。我们的研究表明，在某些特殊条件下，两种方法的分离潜力相当，在4小时内可产生2.5至3.0×10⁹个粒细胞。如果在采血前16小时对供体进行地塞米松治疗，粒细胞采集率最佳。然而，CFC的成本比FL高出约两倍。通过重复过滤洗脱白细胞单采术将细胞损伤降至最低，可使因输注FL粒细胞导致的副作用发生率增加降低至CFC粒细胞的水平。这些研究确定了CFC的效率范围，除了粒细胞分离外，还包括血小板采集、免疫治疗细胞采集和血浆置换。
