Noguer Thierry, Tencaliec Anca, Calas-Blanchard Carole, Avramescu Alina, Marty Jean-Louis
Université de Perpignan, Centre de Phytopharmacie, UMR CNRS 5054, 52, Perpignan, France.
J AOAC Int. 2002 Nov-Dec;85(6):1382-9.
A monoenzymatic amperometric biosensor was developed for the detection of acetaldehyde. The sensor is based on the association of screen-printed carbon electrodes and aldehyde dehydrogenase immobilized by a sol-gel entrapment method. Modification of screen-printed carbon electrodes with Reinecke salt of Meldola's Blue (MBRS) resulted in highly sensitive and interference-free nicotinamide-adenine dinucleotide (NADH) detectors. Based on MBRS-mediated oxidation of NADH at -150 mV versus pseudo Ag/AgCl, acetaldehyde was determined in the range 10-260 microM, compatible with wine quality monitoring. The method of immobilization based on sol-gel entrapment was optimized to obtain the best compromise between sensitivity and operational stability. The sensor response was stable for 40 consecutive assays with methyltrimethoxysilane used as alkoxide precursor, thus allowing a possible calibration of the sensor before each measurement. The biosensors were used to analyze French wines. The method was validated with a commercially available enzymatic kit based on a standard spectrophotometric method.
开发了一种用于检测乙醛的单酶安培生物传感器。该传感器基于丝网印刷碳电极与通过溶胶 - 凝胶包埋法固定的乙醛脱氢酶的结合。用麦尔多拉蓝雷氏盐（MBRS）修饰丝网印刷碳电极，得到了高灵敏度且无干扰的烟酰胺腺嘌呤二核苷酸（NADH）检测器。基于在相对于伪Ag/AgCl为 -150 mV时MBRS介导的NADH氧化，在10 - 260 microM范围内测定乙醛，这与葡萄酒质量监测兼容。优化了基于溶胶 - 凝胶包埋的固定方法，以在灵敏度和操作稳定性之间取得最佳平衡。以甲基三甲氧基硅烷作为醇盐前体时，传感器响应在连续40次测定中保持稳定，因此允许在每次测量前对传感器进行校准。这些生物传感器用于分析法国葡萄酒。该方法通过基于标准分光光度法的市售酶试剂盒进行了验证。
