Whiteside Theresa L
University of Pittsburgh Cancer Institute, 200 Lothrop Street, BST 211, W1041, Pittsburgh, PA 15213-2582, USA.
Vaccine. 2002 Dec 19;20 Suppl 4:A46-51. doi: 10.1016/s0264-410x(02)00387-0.
Rapid turnover of lymphocytes observed in patients with cancer appears to be driven by increased apoptosis of T lymphocytes or insufficient thymic output of recent thymic emigrants (RTE). Using multicolor flow cytometry and apoptosis assays, we found that CD8+CD95+Annexin+ T cells are dying at a rate that is significantly higher in patients with cancer than in normal controls (NC). CD8+ effector subsets of T cells were particularly vulnerable to apoptosis. Thymic excision circle (TREC) analysis of peripheral blood lymphocytes showed a decreased number of RTE in these patients. Together, the data suggest that a high rate of T-cell turnover might contribute to immunologic imbalance in patients with cancer and have unfavorable effects on immunotherapy, including therapeutic antitumor vaccines.
在癌症患者中观察到的淋巴细胞快速更新似乎是由T淋巴细胞凋亡增加或近期胸腺迁出者（RTE）的胸腺输出不足所驱动。使用多色流式细胞术和凋亡检测，我们发现CD8 + CD95 +膜联蛋白+ T细胞的死亡速率在癌症患者中显著高于正常对照（NC）。T细胞的CD8 +效应子集尤其容易发生凋亡。对外周血淋巴细胞的胸腺切除环（TREC）分析显示这些患者中RTE的数量减少。总之，数据表明T细胞的高更新率可能导致癌症患者的免疫失衡，并对免疫治疗，包括治疗性抗肿瘤疫苗产生不利影响。
