Chavagnat Frederic, Haueter Monika, Jimeno Juan, Casey Michael G
Department of Microbiology, Swiss Dairy Research Institute, Liebefeld, CH-3003 Bern, Switzerland.
FEMS Microbiol Lett. 2002 Dec 17;217(2):177-83. doi: 10.1111/j.1574-6968.2002.tb11472.x.
Comparative analysis of partial tuf sequences was evaluated for the identification and differentiation of lactobacilli. Comparison of the amino acid sequences allowed differentiation between species and also between the subspecies of Lactobacillus delbrueckii. The nucleotide sequence comparison allowed differentiation between other subspecies and between some strains. Lactobacilli from several collections and isolates from dairy samples were clearly identified by comparison of short tuf sequences with those of the type strains. In evaluating the taxonomy of the Lactobacillus casei-related taxa, different tuf amino acid signatures are in favour of a classification into three distinct species. The type strain designation for the L. casei species is discussed.
对部分tuf序列进行比较分析，以鉴定和区分乳酸杆菌。氨基酸序列的比较有助于区分不同的物种以及德氏乳杆菌的不同亚种。核苷酸序列的比较有助于区分其他亚种和一些菌株。通过将短tuf序列与模式菌株的序列进行比较，可清晰鉴定来自多个菌种保藏中心的乳酸杆菌以及乳制品样品中的分离株。在评估干酪乳杆菌相关分类群的分类学时，不同的tuf氨基酸特征支持将其分为三个不同的物种。文中还讨论了干酪乳杆菌种的模式菌株指定问题。
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