Bioresource Collection and Research Center, Food Industry Research and Development Institute, 331 Shih-Pin Road, Hsinchu 30062, Taiwan, ROC.
Mol Cell Probes. 2014 Feb;28(1):31-3. doi: 10.1016/j.mcp.2013.07.004. Epub 2013 Aug 19.
The aim of this study was to use tuf gene as a molecular target for species discrimination in the Acetobacter genus, as well as to develop species-specific PCR method for direct species identification of Acetobacter aceti. The results showed that most Acetobacter species could be clearly distinguished, and the average sequence similarity for the tuf gene (89.5%) among type strains was significantly lower than that of the 16S rRNA gene sequence (98.0%). A pair of species-specific primers were designed and used to specifically identify A. aceti, but none of the other Acetobacter strains. Our data indicate that the phylogenetic relationships of most strains in the Acetobacter genus can be resolved using tuf gene sequencing, and the novel species-specific primer pair could be used to rapidly and accurately identify the species of A. aceti by the PCR based assay.
本研究旨在将 tuf 基因作为醋酸杆菌属种间区分的分子靶标，并建立用于醋酸杆菌属中醋酸杆菌（Acetobacter aceti）直接种鉴定的种特异性 PCR 方法。结果表明，大多数醋酸杆菌种可被明确区分，tuf 基因（89.5%）的平均序列相似性显著低于 16S rRNA 基因序列（98.0%）。设计并使用一对种特异性引物可特异性鉴定 A. aceti，但不能鉴定其他醋酸杆菌菌株。我们的数据表明，使用 tuf 基因测序可以解析醋酸杆菌属中大多数菌株的系统发育关系，新型种特异性引物对可通过基于 PCR 的检测方法快速准确地鉴定 A. aceti 的种。
