肠道抑菌性乳酸菌的筛选及其抑菌物质的研究。
Screening beneficial bacteriostatic lactic acid bacteria in the intestine and studies of bacteriostatic substances.
机构信息
Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, China.
College of Food Science, Northeast Agricultural University, Harbin 150030, China.
出版信息
J Zhejiang Univ Sci B. 2021 Jul 15;22(7):533-547. doi: 10.1631/jzus.B2000602.
Lactic acid bacteria (LAB) are a representative probiotic. As the dominant flora in the human intestinal tract, LAB can regulate the balance of human intestinal flora and improve host health. The purpose of this study was to isolate and screen LAB that are well suited to the intestinal characteristics of the Chinese population, with excellent probiotics and high antibacterial activity. After 16S ribosomal RNA (rRNA) homology and phylogenetic tree analysis, potential probiotics were tested for their antibacterial activity, resistance to artificial gastrointestinal fluid and drugs, surface hydrophobicity, and safety. Three strains of LAB with acid resistance, bile salt resistance, epithelial cell adhesion, and no multidrug resistance were selected: , , and . Analysis of the antibacterial active substances in the three strains and their fermentation broths revealed that the main antibacterial substances of were organic acids, whereas those of and were organic acids and bacteriocins with broad-spectrum antibacterial activity. These three strains of probiotic LAB with high antibacterial activity were identified as bacterial resources that could potentially be used to develop probiotic preparations for the prevention and treatment of intestinal diseases caused by intestinal pathogens.
乳酸菌（LAB）是一种代表性的益生菌。作为人类肠道中的优势菌群，LAB 可以调节人体肠道菌群的平衡，改善宿主健康。本研究旨在分离和筛选出适合中国人群肠道特点、具有优良益生菌特性和高抗菌活性的 LAB。通过 16S 核糖体 RNA（rRNA）同源性和系统发育树分析，对潜在的益生菌进行抗菌活性、耐人工胃肠液和药物、表面疏水性和安全性测试。筛选出 3 株具有耐酸、耐胆盐、黏附上皮细胞和无多重耐药性的 LAB 菌株： 、 、 。对 3 株菌及其发酵液中的抗菌活性物质进行分析，结果表明 的主要抗菌物质为有机酸， 和 的主要抗菌物质为具有广谱抗菌活性的有机酸和细菌素。这 3 株具有高抗菌活性的益生菌 LAB 被鉴定为细菌资源，可用于开发预防和治疗由肠道病原体引起的肠道疾病的益生菌制剂。
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