Bayard B, Fournet B
Carbohydr Res. 1976 Jan;46(1):75-86. doi: 10.1016/s0008-6215(00)83532-6.
Hydrazinolysis followed by nitrous acid deamination of alpha1-acid glycoprotein gave acidic and neutral mono- and oligo-saccharides that contain 2,5-anhydro-D-mannose as reducing residue: alpha-D-Manp-(1 leads to 3)-[alpha-D-Manp-(1 leads to 6)]-beta-D-Manp-(1 leads to 4)-2,5-anhydro D-mannose (1), beta D-Galp-(1 leads to 4)-2,5-anhydro-D-mannose (3), 2,5-anhydro-D-mannose, and two N-acetylneuraminic acid-containing oligosaccharides having the common partial sequence: NeuNAc-(2 leads to ?)-[BETA-D-Galp-(1 leads to 4)-2,5-anhydro-D-mannose] (5). This specific cleavage of 2-amino-s-deoxy-D-glucosyl linkages released almost quantitatively a very limited number of saccharides. Reduction with sodium borotritide of the products of cleavage allowed the precise determination of the molar proportion of 1, 3, and free 2,5-anhydro-D-mannose.
对α1-酸性糖蛋白进行肼解，然后用亚硝酸脱氨，得到酸性和中性的单糖和寡糖，这些糖含有2,5-脱水-D-甘露糖作为还原端：α-D-甘露糖基-(1→3)-[α-D-甘露糖基-(1→6)]-β-D-甘露糖基-(1→4)-2,5-脱水-D-甘露糖(1)、β-D-半乳糖基-(1→4)-2,5-脱水-D-甘露糖(3)、2,5-脱水-D-甘露糖，以及两种含有N-乙酰神经氨酸的寡糖，它们具有共同的部分序列：NeuNAc-(2→?)-β-D-半乳糖基-(1→4)-2,5-脱水-D-甘露糖。2-氨基-2-脱氧-D-葡糖基键的这种特异性裂解几乎定量地释放出数量非常有限的糖类。用硼氢化三氚钠还原裂解产物，可以精确测定1、3和游离2,5-脱水-D-甘露糖的摩尔比例。
