Baussant T, Alonso C, Wieruszeski J M, Strecker G, Montreuil J, Alhenc-Gelas F, Michalski J C
Unité 36 I.N.S.E.R.M., Paris, France.
Biochem J. 1992 Apr 15;283 ( Pt 2)(Pt 2):531-5. doi: 10.1042/bj2830531.
Rat T-kininogen has been separated into two molecular variants by affinity chromatography on concanavalin A (ConA): a ConA-reactive (ConA+) and a ConA-non-reactive (ConA-) fraction, from which carbohydrate chains were quantitatively released by hydrazinolysis. On the basis of high-resolution 400 MHz 1H-n.m.r. spectroscopy of the re-N-acetylated hydrazinolysates, the carbohydrate structures of the two ConA molecular variants of rat T-kininogen were established. The ConA-non-reactive species contains a single type of carbohydrate chain with the following structure: [formula: see text] The ConA-reactive fraction contains the same structure and the following additional one: [formula: see text] The relative abundance of the two molecular forms is profoundly affected during inflammation (ratio ConA+/ConA-: 44% in normal and 95% in inflamed T-kininogen), but no structural modification of the carbohydrate chains was observed.
通过伴刀豆球蛋白A（ConA）亲和层析，大鼠T-激肽原已被分离为两种分子变体：一种ConA反应性（ConA+）和一种ConA非反应性（ConA-）组分，通过肼解从其中定量释放出碳水化合物链。基于对重新N-乙酰化肼解产物的高分辨率400 MHz 1H-核磁共振光谱，确定了大鼠T-激肽原两种ConA分子变体的碳水化合物结构。ConA非反应性物种含有单一类型的具有以下结构的碳水化合物链：[化学式：见正文]。ConA反应性组分含有相同结构以及以下额外结构：[化学式：见正文]。在炎症过程中，两种分子形式的相对丰度受到深刻影响（ConA+/ConA-比例：正常T-激肽原中为44%，炎症T-激肽原中为95%），但未观察到碳水化合物链的结构修饰。
