Osaka Takashi, Beika Asa, Hattori Asuka, Kohno Yoshinori, Kato Koichi H, Mizutani Takaharu
Graduate School of Pharmaceutical Sciences, Nagoya City University, Japan.
Biochem Biophys Res Commun. 2003 Jan 3;300(1):236-40. doi: 10.1016/s0006-291x(02)02806-1.
In the phylogenetic tree, selenoproteins and the corresponding translation machinery are found in Archaea, Eubacteria, and animals, but not in fungi and higher plants. As very little is known about Protozoa, we searched for the presence of selenoproteins in the primitive dinoflagellate Oxyrrhis marina, belonging to the Protoctista kingdom. Four selenoproteins could be obtained from O. marina cells cultured in the presence of 75Se. Using O. marina or bovine liver cytosolic extracts, we could serylate and selenylate in vitro total O. marina tRNAs. Moreover, the existence of a tRNA(Sec) could be deduced from in vivo experiments. Lastly, an anti-serum against the specialized mammalian translation elongation factor mSelB reacted with a protein of 48-kDa molecular mass. Altogether, our data showed that O. marina contains selenoproteins and suggests that the corresponding translation machinery is related to that found in animals.
