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人类带型染色体的显微光度测定法。带型模式的显微摄影技术。

Microphotometry of banded human chromosomes II. Technique for microphotography of banding patterns.

作者信息

Lundsteen C

出版信息

Clin Genet. 1976 Feb;9(2):156-62. doi: 10.1111/j.1399-0004.1976.tb01562.x.

DOI:10.1111/j.1399-0004.1976.tb01562.x
PMID:1248174
Abstract

As part of a study of chromosome banding patterns by microphotometric measurements of photographic negatives, banded chromosomes were microphotographed in order to evaluate the importance of the size of the condensor (illumination) aperture for obtaining high resolution. The resolution was found to be closely correlated to the numerical aperture of the objective, but within wide limits it was independent of the condensor aperture. The reason is assumed to be light scattering caused by the object, which results in illumination of the full objective aperture. The optimal photographic method was found to include the use of a 63x oil planapochromate objective (NA 1.4) and a total microscope magnification of 250x.

摘要

作为一项通过对摄影底片进行显微光度测量来研究染色体带型模式的研究的一部分，对带型染色体进行了显微拍照，以评估聚光镜（照明）孔径大小对于获得高分辨率的重要性。结果发现分辨率与物镜的数值孔径密切相关，但在很大范围内它与聚光镜孔径无关。据推测，原因是物体引起的光散射，这导致整个物镜孔径被照亮。发现最佳的摄影方法包括使用63x油浸平场复消色差物镜（数值孔径1.4）和250倍的总显微镜放大倍数。

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