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人类带型染色体的显微光度测定。与在显微照片上扫描相比，通过直接扫描标本可获得高分辨力。

Microphotometry of banded human chromosomes. High resolving power by direct scanning of the specimen compared with scanning on microphotographs.

作者信息

Lundsteen C, Philip J

出版信息

J Med Genet. 1974 Jun;11(2):196-200. doi: 10.1136/jmg.11.2.196.

DOI:10.1136/jmg.11.2.196
PMID:4135402
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1013118/
Abstract

A method which was designed for microphotometric measurements of banded human chromosomes using a computer-controlled Zeiss scanning microscope photometer (SMP) is described. Modification of the Apamos 2 program (H. G. Zimmer, Zeiss, Oberkochen) enabled the scanning stage on the SMP to move the slides continuously thus obtaining high resolution of measurements. Results of scanning directly on the slides when compared with scanning on microphotographs revealed no significant differences; the banding patterns of the chromosomes were reproduced with a high degree of accuracy by both methods. Chromosomes No. 2, 10, 16, and 20 are described in detail.

摘要

本文描述了一种使用计算机控制的蔡司扫描显微镜光度计（SMP）对人类带型染色体进行显微光度测量的方法。对Apamos 2程序（H.G.齐默，蔡司，奥伯科亨）的修改使SMP上的扫描台能够连续移动载玻片，从而获得高分辨率的测量结果。与在显微照片上扫描相比，直接在载玻片上扫描的结果显示没有显著差异；两种方法都能高度准确地再现染色体的带型模式。详细描述了2号、10号、16号和20号染色体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f88/1013118/dc6a28102a32/jmedgene00319-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f88/1013118/a6fdb6a1428c/jmedgene00319-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f88/1013118/dc6a28102a32/jmedgene00319-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f88/1013118/a6fdb6a1428c/jmedgene00319-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f88/1013118/dc6a28102a32/jmedgene00319-0090-a.jpg

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本文引用的文献

1
Chromosome preparations of leukocytes cultured from human peripheral blood.从人外周血培养的白细胞的染色体标本制备。
Exp Cell Res. 1960 Sep;20:613-6. doi: 10.1016/0014-4827(60)90138-5.
2
Automatic karyotyping of quinacrine mustard stained human chromosomes.喹吖因芥子染色人类染色体的自动核型分析。
Exp Cell Res. 1971 Jul;67(1):233-5. doi: 10.1016/0014-4827(71)90645-8.
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Identification of human chromosome regions by aid of computerized pattern analysis.
Exp Cell Res. 1972 Feb;70(2):475-8. doi: 10.1016/0014-4827(72)90171-1.
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The fluorescent pattern of normal chromosomes in biopsies of malignant lymphomas, and its computer display.恶性淋巴瘤活检中正常染色体的荧光模式及其计算机显示。
Hereditas. 1972;70(1):75-88. doi: 10.1111/j.1601-5223.1972.tb00995.x.
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Microspectrophotometry of trypsin-Leishman-stained human chromosomes.胰蛋白酶-利什曼染色的人类染色体的显微分光光度测定法。
Nature. 1973 Apr 27;242(5400):613-5. doi: 10.1038/242613a0.
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Computerized chromosome identification by aid of the quinacrine mustard fluorescence technique.借助喹吖因芥子荧光技术进行计算机化染色体识别。
Hereditas. 1972;67(1):103-10. doi: 10.1111/j.1601-5223.1971.tb02364.x.
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A rapid banding technique for human chromosomes.一种用于人类染色体的快速显带技术。
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