Lisdiyanti Puspita, Kawasaki Hiroko, Seki Tatsuji, Yamada Yuzo, Uchimura Tai, Komagata Kazuo
Laboratory of General and Applied Microbiology, Department of Applied Biology and Chemistry, Faculty of Applied Bioscience, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan.
J Gen Appl Microbiol. 2001 Jun;47(3):119-131. doi: 10.2323/jgam.47.119.
Forty-six strains of acetic acid bacteria newly isolated from flowers, fruits, and fermented foods collected in Indonesia were taxonomically studied. They were Gram-negative rods, produced acetic acid from ethanol, oxidized acetate and lactate to CO(2) and H(2)O, and had Q-9 as the major ubiquinone system. On the basis of DNA-DNA similarity, all strains studied, including type strains and reference strains of the genus Acetobacter, were separated into eleven groups (Groups I to XI). Of the 46 isolates, two isolates were included in Group II and identified as Acetobacter pasteurianus, five in Group IV as A. orleanensis, 16 in Group V as A. lovaniensis, five in Group VII as A. indonesiensis, and three in Group VIII as A. tropicalis. The remaining 15 isolates constituted three new groups based on DNA-DNA similarity; four isolates were included in Group IX, two in Group X, and nine in Group XI. No isolates were identified as A. aceti (Group I), A. peroxydans (Group III), and A. estunensis (Group VI). Phylogenetic analysis based on 16S rDNA sequences of representative strains of the Groups indicated belonging to the strains of the genus Acetobacter. On the basis of DNA base composition, DNA-DNA similarity, and 16S rDNA sequences, three new species of the genus Acetobacter are proposed: Acetobacter syzygii sp. nov. for Group IX, Acetobacter cibinongensis sp. nov. for Group X, and Acetobacter orientalis sp. nov. for Group XI. The distribution of Acetobacter strains in Indonesia is discussed in light of isolation sources.
对从印度尼西亚采集的花卉、水果和发酵食品中新分离出的46株醋酸菌进行了分类学研究。它们为革兰氏阴性杆菌，能将乙醇转化为乙酸，将乙酸盐和乳酸盐氧化为二氧化碳和水，并且以Q-9作为主要的泛醌系统。基于DNA-DNA相似性，所有研究菌株，包括醋酸杆菌属的模式菌株和参考菌株，被分为11个组（I组至XI组）。在这46株分离菌中，有2株归入II组，鉴定为巴斯德醋酸杆菌；5株归入IV组，为奥尔良醋酸杆菌；16株归入V组，为洛凡尼醋酸杆菌；5株归入VII组，为印度尼西亚醋酸杆菌；3株归入VIII组，为热带醋酸杆菌。其余15株分离菌根据DNA-DNA相似性构成了三个新组；4株归入IX组，2株归入X组，9株归入XI组。未鉴定出有分离菌属于醋化醋酸杆菌（I组）、过氧化醋酸杆菌（III组）和埃斯图嫩斯醋酸杆菌（VI组）。基于这些组代表性菌株的16S rDNA序列的系统发育分析表明这些菌株属于醋酸杆菌属。根据DNA碱基组成、DNA-DNA相似性和16S rDNA序列，提出了醋酸杆菌属的三个新种：IX组的合子醋酸杆菌新种、X组的芝宾翁醋酸杆菌新种和XI组的东方醋酸杆菌新种。根据分离来源讨论了醋酸杆菌菌株在印度尼西亚的分布情况。
