Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, 254 Phayathai Road, Wangmai, Bangkok 10330, Thailand.
Microbes Environ. 2009;24(2):135-43. doi: 10.1264/jsme2.me08564.
Twenty-six strains of acetic acid bacteria were isolated from fruits, flowers and related materials collected in Thailand. They were divided into three genera, Acetobacter, Gluconobacter and Asaia, by phenotypic characterization and 16S rRNA gene sequence analyses. On the basis of 16S-23S rRNA gene internal transcribed spacer (16S-23S rDNA ITS) restriction and 16S rRNA gene sequence analyses, fourteen isolates assigned to the genus Acetobacter were divided into five groups: 1) Group 1A or A. tropicalis (one isolate); 2) Group 2A or A. orientalis (four isolates); 3) Group 3A or A. pasteurianus (five isolates); 4) Group 4A or A. syzygii (one isolate); and 5) Group 5A or A. ghanensis (three isolates). The eleven isolates assigned to the genus Gluconobacter were divided into three groups: 6) Group 1B or G. frateurii (four isolates); 7) Group 2B or G. japonicus (six isolates); and 8) Group 3B or unidentified (one isolate). The remaining isolate was placed into: 9) Group 1C or unidentified, which was assigned to the genus Asaia and considered to constitute a new species on the basis of the 16S rRNA gene sequence analysis and DNA-DNA hybridization.
从泰国采集的水果、花卉及相关材料中分离到 26 株醋酸菌。通过表型特征和 16S rRNA 基因序列分析，将它们分为 3 个属，即醋酸杆菌属、葡萄糖酸杆菌属和亚洲酸杆菌属。基于 16S-23S rRNA 基因内转录间隔区（16S-23S rDNA ITS）的限制和 16S rRNA 基因序列分析，将 14 株归属于醋酸杆菌属的菌株分为 5 个组：1）组 1A 或 A. tropicalis（1 株）；2）组 2A 或 A. orientalis（4 株）；3）组 3A 或 A. pasteurianus（5 株）；4）组 4A 或 A. syzygii（1 株）；5）组 5A 或 A. ghanensis（3 株）。11 株归属于葡萄糖酸杆菌属的菌株分为 3 个组：6）组 1B 或 G. frateurii（4 株）；7）组 2B 或 G. japonicus（6 株）；8）组 3B 或未鉴定（1 株）。其余的菌株被归入：9）组 1C 或未鉴定，该菌株基于 16S rRNA 基因序列分析和 DNA-DNA 杂交，被归属于亚洲酸杆菌属，被认为是一个新种。
