Sokollek S J, Hertel C, Hammes W P
Institut für Lebensmitteltechnologie, Universität Hohenheim, Stuttgart, Germany.
Int J Syst Bacteriol. 1998 Jul;48 Pt 3:935-40. doi: 10.1099/00207713-48-3-935.
Two strains of Acetobacter sp., LTH 2460T and LTH 2458T, have been isolated from running red wine and cider vinegar fermentations, respectively. Taxonomic characteristics of the isolates were investigated. Comparative analysis of the 165 rRNA sequences revealed > 99% similarity between strain LTH 2460T and the type strains of the related species Acetobacter europaeus and Acetobacter xylinus and between strain LTH 2458T and Acetobacter pasteurianus. On the other hand, low levels of DNA relatedness (< 34%) were determined in DNA-DNA similarity studies. This relatedness below the species level was consistent with specific physiological characteristics permitting clear identification of these strains within established species of acetic acid bacteria. Based on these results, the names Acetobacter oboediens sp. nov. and Acetobacter pomorum sp. nov. are proposed for strains LTH 2460T and LTH 2458T, respectively. The phylogenetic positions of the new species are reflected by a 16S rRNA-based tree. Furthermore, a 16S rRNA-targeted oligonucleotide probe specific for A. oboediens was constructed.
分别从正在进行的红酒和苹果醋发酵过程中分离出了两株醋酸杆菌属菌株，即LTH 2460T和LTH 2458T。对这些分离菌株的分类学特征进行了研究。16S rRNA序列的比较分析表明，LTH 2460T菌株与相关物种欧洲醋酸杆菌和木醋杆菌的模式菌株之间的相似度> 99%，LTH 2458T菌株与巴氏醋酸杆菌之间的相似度也> 99%。另一方面，在DNA - DNA相似性研究中确定了较低水平的DNA相关性（< 34%）。这种低于物种水平的相关性与特定的生理特征一致，这些生理特征使得能够在已确定的醋酸菌物种内明确鉴定这些菌株。基于这些结果，分别为LTH 2460T和LTH 2458T菌株提议了新种名顺从醋酸杆菌（Acetobacter oboediens sp. nov.）和苹果醋酸杆菌（Acetobacter pomorum sp. nov.）。新物种的系统发育位置通过基于16S rRNA的树状图得以体现。此外，构建了一种针对顺从醋酸杆菌的16S rRNA靶向寡核苷酸探针。
