Caceres C
Entomology Unit, FAO/IAEA Agriculture and Biotechnology Laboratory, A-2444, Seibersdorf, Austria.
Genetica. 2002 Sep;116(1):107-16. doi: 10.1023/a:1020967810703.
Genetic sexing strains (GSS) based on the temperature sensitive lethal (tsl) mutation are being used to produce sterile male medflies for large scale sterile insect technique (SIT) programmes for this pest. The use of male-only strains increases the overall efficiency of the technique. Currently more than 1.4 billion sterile male-only pupae are produced per week in different facilities around the world. Due to the mutations used to construct these strains, that is, translocations and selectable markers, they require different and more careful mass rearing procedures than do bisexual strains (BSS). The basic rearing technology has been developed and can be used to produce only males on a predictable basis to a level of 99.9% accuracy. If specific rearing procedures are followed, then tsl-based GSS has a rearing efficiency that is equal to that of a BSS and it is already know that males produced by the tsl-based GSS are of equal quality to males produced by BSS. Based on current rearing technology the cost of production of male pupae is about the same for both types of strain. This is due to the large colony that is required for the tsl-based GSS. This paper discusses the considerations that need to be taken into account during mass rearing of GSS and identifies the most efficient production processes that are currently available.
基于温度敏感致死（tsl）突变的遗传性别品系（GSS）正被用于为这种害虫的大规模不育昆虫技术（SIT）项目生产不育雄地中海实蝇。仅使用雄性品系可提高该技术的整体效率。目前，世界各地不同设施每周生产超过14亿只仅为雄性的不育蛹。由于用于构建这些品系的突变，即易位和选择标记，与双性品系（BSS）相比，它们需要不同且更精细的大规模饲养程序。基本饲养技术已经开发出来，可用于在可预测的基础上仅生产雄性，准确率达到99.9%。如果遵循特定的饲养程序，那么基于tsl的GSS的饲养效率与BSS相当，并且已经知道基于tsl的GSS生产的雄性与BSS生产的雄性质量相同。基于当前的饲养技术，两种品系雄性蛹的生产成本大致相同。这是由于基于tsl的GSS需要大量的种群。本文讨论了在GSS大规模饲养过程中需要考虑的因素，并确定了目前可用的最有效的生产流程。
