Kiuchi Katsuji, Yoshizawa Katsuhiko, Moriguchi Kaei, Tsubura Airo
Department of Pathology, Kansai Medical University, Moriguchi, Osaka, Japan.
Exp Toxicol Pathol. 2002 Nov;54(3):181-6. doi: 10.1078/0940-2993-00253.
Cataract was induced by a single intraperitoneal injection of N-methyl-N-nitrosourea (MNU) in 15-day-old Sprague-Dawley (Jcl: SD) rats. The threshold dose of MNU required for cataract induction in a 3-4 week time period was 70 mg/kg; 60 mg/kg was ineffective. Males and females were both equally affected. Mature cataract as confirmed histologically by degeneration, swelling, vacuolation, liquefaction of the lens fibers, and formation of Morgagni-like water vacuoles was seen in 80% (8/10), 70% (7/10) and 90% (9/10) of 100, 80 and 70 mg/kg MNU-treated rats, respectively, 4 weeks after dosing (43 days of age). At this time point, lens epithelial apoptosis was only rarely seen, but proliferating cell nuclear antigen (PCNA) labeled atypical nuclei with vacuolated lens fibers and macrophage migration were present within the injured lens. Cataracts were the only lesions induced by MNU and there were no other intra- or extraocular lesions seen. The dosing and timing schedule for the MNU administration in rats used in the present study is effective in rapidly causing cataract to occur.
通过对15日龄的斯普拉格-道利（Jcl: SD）大鼠腹腔注射一次N-甲基-N-亚硝基脲（MNU）来诱导白内障。在3至4周的时间段内诱导白内障所需的MNU阈值剂量为70毫克/千克；60毫克/千克无效。雄性和雌性受影响程度相同。给药4周后（43日龄），在分别接受100、80和70毫克/千克MNU处理的大鼠中，80%（8/10）、70%（7/10）和90%（9/10）出现了经组织学证实的成熟白内障，表现为晶状体纤维变性、肿胀、空泡化、液化以及形成类莫尔加尼水空泡。此时，仅很少见到晶状体上皮细胞凋亡，但在受损晶状体中存在增殖细胞核抗原（PCNA）标记的非典型细胞核、有空泡的晶状体纤维以及巨噬细胞迁移。白内障是MNU诱导产生的唯一病变，未观察到其他眼内或眼外病变。本研究中用于大鼠的MNU给药剂量和时间安排能有效快速诱发白内障。
