Roy B, Fujimoto N, Watanabe H, Ito A
Hiroshima J Med Sci. 1989 Sep;38(3):95-8.
Cataracts were observed in female F344 rats who intravenously received methylnitrosourea (MNU), a potent carcinogen for multiple organs, in one dose of 50 mg/kg body weight. Induction of cataract 40 weeks after MNU treatment was 41% whereas no cataract was observed in control rats. The aggregation of beta H-crystallin fraction by MNU was studied in vitro. HPLC pattern of beta H-crystallin changed when lens protein was incubated for 24 hrs with MNU. HPLC patterns indicated that MNU induced high molecular weight aggregates of beta H-crystallin. This study conveys some indication about the direct interaction of MNU with lens protein in cataract formation.
在静脉注射甲基亚硝基脲（MNU，一种对多个器官有强致癌性的物质）、剂量为50毫克/千克体重的雌性F344大鼠中观察到了白内障。MNU治疗40周后白内障的诱发率为41%，而在对照大鼠中未观察到白内障。对MNU诱导βH-晶状体蛋白组分聚集进行了体外研究。当晶状体蛋白与MNU孵育24小时时，βH-晶状体蛋白的高效液相色谱（HPLC）图谱发生了变化。HPLC图谱表明，MNU诱导了βH-晶状体蛋白形成高分子量聚集体。这项研究为MNU在白内障形成过程中与晶状体蛋白的直接相互作用提供了一些线索。
