Meager A, Butters T D, Mautner V, Hughes R C
Eur J Biochem. 1976 Jan 15;61(2):345-53. doi: 10.1111/j.1432-1033.1976.tb10028.x.
Glycoprotein material extracted from human KB cells with a flurocarbon, trichlorotrifluoroethane (Arklone P), into a waste-soluble fraction binds to fibre, a structural protein of the adenovirus type-5 capsid. The fibre-binding glycoprotein(s) were purified by ion-exchange chromatography on DEAE-Sephadex and affinity chromatography on a fibre-Sepharose support. The purification procedure also includes a trypsinization step which eliminates the bulk of contaminating KB cell proteins present in the aqueous fraction without appreciably affecting the activity of the fibre-binding glycoprotein(s). Some comparison is made of the membrane-bound receptors for adenovirus and the water-soluble fibre-binding glycoprotein(s).
用氟碳化合物三氯三氟乙烷（Arklone P）从人KB细胞中提取的糖蛋白物质进入一种可溶于废物的组分，该组分可与纤维结合，纤维是5型腺病毒衣壳的一种结构蛋白。通过在DEAE-葡聚糖上进行离子交换色谱和在纤维-琼脂糖支持物上进行亲和色谱，对纤维结合糖蛋白进行了纯化。纯化过程还包括一个胰蛋白酶处理步骤，该步骤可去除水相组分中大量的污染性KB细胞蛋白，而不会明显影响纤维结合糖蛋白的活性。对腺病毒的膜结合受体和水溶性纤维结合糖蛋白进行了一些比较。
