Ogier G, Michal Y, Thomas V, Quash G, Rodwell J D
Laboratoire d'Immunochimie Inserm, Faculté de Médecine Lyon Sud, Université Claude Bernard Lyon I, Oullins, France.
Arch Virol. 1994;135(1-2):43-60. doi: 10.1007/BF01309764.
Periodate oxidation of purified type 5 Adenovirus (Ad5) led to a mean loss of infectivity of 6.84 logs. There were no significant differences in adsorption and penetration between oxidized and mock-oxidized virus. However, after infection with oxidized virus, no synthesis of viral structural proteins could be detected and a 78.5% inhibition of viral DNA synthesis was observed. Labelling experiments performed by treating oxidized and mock-oxidized virus with tritiated sodium borohydride revealed that the fiber glycoprotein was one of the proteins labelled in oxidized virus whereas no labelled proteins were detected in non oxidized virus. In addition, it was found that one mol of formaldehyde generated during oxidation of sugar residues was bound per 500 base pairs in oxidized virus. One consequence of this in situ generation of formaldehyde is the formation of DNA-protein crosslinks. The DNA so crosslinked showed different patterns of restriction fragments with endonucleases such as Hpa I, Hind III and Kpn I but not with Xho I.
纯化的5型腺病毒（Ad5）经高碘酸盐氧化后，平均感染力损失6.84个对数。氧化病毒与模拟氧化病毒在吸附和穿透方面无显著差异。然而，用氧化病毒感染后，未检测到病毒结构蛋白的合成，且观察到病毒DNA合成受到78.5%的抑制。用氚化硼氢化钠处理氧化病毒和模拟氧化病毒进行的标记实验表明，纤维糖蛋白是氧化病毒中被标记的蛋白之一，而在未氧化病毒中未检测到被标记的蛋白。此外，发现氧化病毒中每500个碱基对结合1摩尔在糖残基氧化过程中产生的甲醛。这种原位产生甲醛的一个后果是形成DNA-蛋白质交联。如此交联的DNA与诸如Hpa I、Hind III和Kpn I等内切核酸酶显示出不同的限制性片段模式，但与Xho I没有。
