在多甲藻素-叶绿素a-蛋白复合体中，类胡萝卜素向叶绿素的能量转移涉及分子内电荷转移态。
Carotenoid to chlorophyll energy transfer in the peridinin-chlorophyll-a-protein complex involves an intramolecular charge transfer state.
作者信息
Zigmantas Donatas, Hiller Roger G, Sundstrom Villy, Polivka Tomas
机构信息
Department of Chemical Physics, Lund University, Box 124, SE-22100 Lund, Sweden.
出版信息
Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16760-5. doi: 10.1073/pnas.262537599. Epub 2002 Dec 16.
Abstract
Carotenoids are, along with chlorophylls, crucial pigments involved in light-harvesting processes in photosynthetic organisms. Details of carotenoid to chlorophyll energy transfer mechanisms and their dependence on structural variability of carotenoids are as yet poorly understood. Here, we employ femtosecond transient absorption spectroscopy to reveal energy transfer pathways in the peridinin-chlorophyll-a-protein (PCP) complex containing the highly substituted carotenoid peridinin, which includes an intramolecular charge transfer (ICT) state in its excited state manifold. Extending the transient absorption spectra toward near-infrared region (600-1800 nm) allowed us to separate contributions from different low-lying excited states of peridinin. The results demonstrate a special light-harvesting strategy in the PCP complex that uses the ICT state of peridinin to enhance energy transfer efficiency.
摘要
类胡萝卜素与叶绿素一样，是光合生物中参与光捕获过程的关键色素。类胡萝卜素向叶绿素能量转移机制的细节及其对类胡萝卜素结构变异性的依赖性目前仍知之甚少。在这里，我们采用飞秒瞬态吸收光谱来揭示含有高度取代的类胡萝卜素——多甲藻素的多甲藻素 - 叶绿素 - a - 蛋白（PCP）复合物中的能量转移途径，该复合物在其激发态多重态中包括一个分子内电荷转移（ICT）态。将瞬态吸收光谱扩展到近红外区域（600 - 1800 nm）使我们能够区分多甲藻素不同低能激发态的贡献。结果表明，PCP复合物中存在一种特殊的光捕获策略，该策略利用多甲藻素的ICT态来提高能量转移效率。
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本文引用的文献
1
Efficient light harvesting through carotenoids.通过类胡萝卜素实现高效光捕获。
Photosynth Res. 2000;66(1-2):125-44. doi: 10.1023/A:1010750332320.
2
An alternative carotenoid-to-bacteriochlorophyll energy transfer pathway in photosynthetic light harvesting.光合光捕获中一种从类胡萝卜素到细菌叶绿素的能量传递替代途径。
Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):6017-22. doi: 10.1073/pnas.092626599. Epub 2002 Apr 23.
3
Carotenoid S(1) state in a recombinant light-harvesting complex of Photosystem II.光系统II重组捕光复合物中的类胡萝卜素S(1)态
Biochemistry. 2002 Jan 15;41(2):439-50. doi: 10.1021/bi011589x.
4
Energy transfer in the peridinin chlorophyll-a protein of Amphidinium carterae studied by polarized transient absorption and target analysis.通过偏振瞬态吸收和靶标分析研究卡特亚得里亚海扁藻中多甲藻叶绿素a蛋白的能量转移
Biophys J. 2001 Jun;80(6):2843-55. doi: 10.1016/S0006-3495(01)76251-0.
5
Carotenoid-to-chlorophyll energy transfer in recombinant major light-harvesting complex (LHCII) of higher plants. I. Femtosecond transient absorption measurements.高等植物重组主要捕光复合体（LHCII）中类胡萝卜素到叶绿素的能量转移。I. 飞秒瞬态吸收测量
Biophys J. 2001 Feb;80(2):901-15. doi: 10.1016/S0006-3495(01)76069-9.
6
Excitation transfer in the peridinin-chlorophyll-protein of Amphidinium carterae.卡氏前沟藻中多甲藻素-叶绿素蛋白的激发转移
Biophys J. 2000 Oct;79(4):1695-705. doi: 10.1016/S0006-3495(00)76422-8.
7
Förster excitation energy transfer in peridinin-chlorophyll-a-protein.在多甲藻叶绿素a蛋白中的Förster激发能量转移
Biophys J. 2000 Jan;78(1):344-53. doi: 10.1016/S0006-3495(00)76597-0.
8
Direct observation of the (forbidden) S1 state in carotenoids.对类胡萝卜素中（禁阻的）S1态的直接观测。
Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):4914-7. doi: 10.1073/pnas.96.9.4914.
9
Carotenoids in photosynthesis.光合作用中的类胡萝卜素。
Photochem Photobiol. 1996 Mar;63(3):257-264. doi: 10.1111/j.1751-1097.1996.tb03022.x.
10
Structural basis of light harvesting by carotenoids: peridinin-chlorophyll-protein from Amphidinium carterae.类胡萝卜素捕光的结构基础：来自卡氏前沟藻的多甲藻叶绿素蛋白复合体
Science. 1996 Jun 21;272(5269):1788-91. doi: 10.1126/science.272.5269.1788.
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