Hanashi Daisuke, Koshino Tomihisa, Uesugi Masaaki, Saito Tomoyuki
Department of Orthopaedic Surgery, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan.
J Orthop Sci. 2002;7(6):672-6. doi: 10.1007/s007760200119.
The purpose of this study was to evaluate the effect of femoral nerve resection on the progression of cartilage degeneration in an anterior cruciate ligament transection (ACLT) and partial meniscectomy (PM) model in rabbits. To induce degeneration of articular cartilage, PM and ACLT were performed on the bilateral knees of 24 rabbits, and femoral sensory nerve selective resection (FNR) was additionally performed on the left knee. Six rabbits each were killed 6 and 10 weeks after surgery to observe early cartilage degeneration and 17 and 24 weeks after surgery to evaluate the late phase. Mankin's histological score at 10 weeks (7.5 +/- 2.7 points; mean +/- SD) after surgery in the FNR group was higher than that at 6 weeks (3.0 +/- 0.6 points) ( P < 0.05). In the control group the score at 17 weeks (7.8 +/- 2.0 points) was higher than that at 6 weeks (2.7 +/- 0.5 points) ( P < 0.05). The concentration of chondroitin sulfate in the joint fluid was quantified by high-performance liquid chromatography. The concentration of chondroitin 6-sulfate in the FNR group at 10 weeks (11.6 +/- 4.1 nmol/ml) was higher than that at the other times and that of the control group at 10 weeks (5.4 +/- 3.2 nmol/ml) after surgery ( P < 0.05). These results indicate that FNR may promote the progression of cartilage degeneration in knees with PM and ACLT.
本研究的目的是评估在兔前交叉韧带横断（ACLT）和部分半月板切除术（PM）模型中，股神经切除对软骨退变进展的影响。为诱导关节软骨退变，对24只兔的双侧膝关节进行了PM和ACLT，并对左膝额外进行了股感觉神经选择性切除（FNR）。分别在术后6周和10周处死6只兔以观察早期软骨退变，在术后17周和24周处死以评估晚期情况。FNR组术后10周的Mankin组织学评分（7.5±2.7分；均值±标准差）高于6周时（3.0±0.6分）（P<0.05）。对照组17周时的评分（7.8±2.0分）高于6周时（2.7±0.5分）（P<0.05）。采用高效液相色谱法定量关节液中硫酸软骨素的浓度。术后10周FNR组硫酸软骨素6 - 硫酸酯的浓度（11.6±4.1 nmol/ml）高于其他时间以及对照组10周时的浓度（5.4±3.2 nmol/ml）（P<0.05）。这些结果表明，FNR可能会促进PM和ACLT膝关节中软骨退变的进展。
